绿色合成的氧化铜纳米颗粒对抗多药耐药细菌的抗菌活性
Toka Khairy1, Dina Hatem Amin2, Hanaa Mohamed Salama3
1Department of Microbiology, Faculty of Science, Ain Shams University, El- Khalyfa El-Mamoun Street, Abbasya, Cairo, Egypt. tokakhairy@sci.asu.edu.eg.
Scientific reports
|October 23, 2024
概括
使用植物提取物合成氧化铜纳米颗粒 (CuO NPs) 的绿色合成显示出显著的抗菌和抗氧化特性. 这些CuONP显示出对抗耐药细菌的替代抗生素的潜力.
科学领域:
- 纳米技术 纳米技术
- 材料科学 材料科学 材料科学
- 绿色化学 绿色化学
背景情况:
- 抗生素耐药性日益增加的威胁需要新的抗菌剂.
- 植物介导的纳米颗粒合成为传统方法提供了一个环保的替代方案.
- 铜氧化物纳米粒子 (CuO NPs) 正在研究其潜在的治疗应用.
研究的目的:
- 合成CuO NPs使用阿扎迪拉赫塔印尼卡和西蒙德西亚chinensis的乙醇提取物.
- 描述合成的CuONP,并评估它们的抗菌,抗氧化和抗菌膜活性.
- 通过分子对接,研究CuO NPs与细菌蛋白质的结合相互作用.
主要方法:
- 使用植物提取物合成CuONP的绿色合成.
- 使用UV-Vis光谱和传输电子显微镜 (TEM) 进行表征.
- 通过阿加扩散和最小抑制度 (MIC) 评估的抗菌活性.
- 进行了抗氧化剂和抗生素膜测试.
- 进行了分子对接研究.
主要成果:
- 通过光谱和显微镜证实了CuONP的成功合成 (尺寸:10.730.9nm).
- 对MRSA,大肠杆菌,P. aeruginosa,Acinetobacter spp.,K. pneumoniae和S. maltophilia具有显著的抗菌活性 (MIC: 62.5125μg/ml). 在这种情况下,它具有很高的抗菌活性.
- 观察到有效的抗氧化剂 (31.192.2%) 和抗菌膜 (62.295%) 活动.
- 分子对接表明CuO NPs与PBP4和OXA-48蛋白质有很强的结合.
结论:
- 通过植物介导合成的CuONP表现出强大的抗菌和抗氧化特性.
- CuO NPs显示为对抗耐药细菌的替代治疗剂具有前途.
- 这项研究支持在制药和医疗保健领域开发新型治疗方法.
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