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Updated: Jul 10, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

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Published on: March 19, 2018

为同时诊断肠道寄生虫,优化18 S rRNA元编码

Dongjun Kang¹, Jun Ho Choi¹, Myungjun Kim¹

¹Department of Tropical Medicine, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea.

Scientific reports

|October 24, 2024

概括

下一代测序成功检测出所有11种肠道寄生虫物种使用18S rDNA V9区域. 诸如DNA二次结构和PCR回火温度等因素影响了寄生虫读数,影响了检测准确度.

科学领域:

医学寄生虫学医学寄生虫学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

下一代测序 (NGS) 为同时检测多种寄生虫物种提供了先进的工具.
18S核糖体DNA (rDNA) V9区域是一个保存的标记物,适合寄生虫识别.

研究的目的:

评估安普利康下一代测序 (NGS) 检测11种肠道寄生虫的有效性.
使用NGS调查影响寄生虫DNA准确量化因素.

主要方法:

从11种肠道寄生虫中克隆18SrDNAV9区域,并将其转化为等离子体.
在Illumina iSeq 100平台上汇集等等量等离子体并执行放大NGS.
分析读数,并将其与DNA二次结构和PCR条件相关联.

主要成果:

检测到所有11个向寄生虫物种,共产生434,849次阅读.
在物种之间观察到读数的显著差异,Clonorchis sinensis和Entamoeba histolytica的比例最高.
在DNA二次结构稳定性和输出读数之间发现了负相关性;PCR回火温度也影响了相对丰度.

结论:

针对18SrDNA V9区域的Amplicon NGS是一种可行的方法,可以同时检测多种肠道寄生虫.
了解DNA二次结构和PCR参数的影响对于优化寄生虫检测和量化至关重要.
这些发现有助于改善肠道寄生虫感染的诊断工具,有助于控制和预防工作.

关键词:

在18S rDNA中.头盔虫是一种虫.肠道寄生虫肠道寄生虫超标标的代码化下一代测序测序是什么原生动物原生动物

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