Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Reporter Genes02:11

Reporter Genes

11.2K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Structural Control of Dual Emission in Coumarin Fluorophores for Visualizing Protein Droplet Maturation.

Angewandte Chemie (International ed. in English)·2026
Same author

Cage-Type Porous Organic Salts as Ship-in-a-Bottle Nanoreactors for Light-Transparent and Reusable Molecular Photocatalysts.

Angewandte Chemie (International ed. in English)·2026
Same author

AI-Guided <i>De Novo</i> Design of a Caffeine-Induced Protein Dissociation System.

Journal of the American Chemical Society·2026
Same author

Phage Display Driven Identification and Computational Mapping of Macrocyclic Peptides Targeting RhoA G17V.

Biochemistry·2026
Same author

Engineering of genetically encoded programmable calcium channel inhibitory binders.

Nature communications·2026
Same author

Kupffer Cell Capture-Evading Modifiable Sub-20 nm Lipid Nanodisc-Based <sup>19</sup>F Magnetic Resonance Imaging Probes.

Journal of the American Chemical Society·2026

相关实验视频

Updated: Jun 9, 2025

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
07:59

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Published on: March 22, 2018

7.6K

光激活基因表达系统使用无格组的可光激活染料

Tatsuki Nonomura1,2, Masafumi Minoshima1,3, Kazuya Kikuchi1,4

  • 1Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1, Yamadaoka, Suita, Osaka, 5650871, Japan.

Angewandte Chemie (International ed. in English)
|October 24, 2024
PubMed
概括

研究人员开发了一种新的光介导系统来控制基因表达. 这种方法使用可光激活染料 (PaX560) 和转录调节器 (QacR) 来实现精确的可见光激活转录,毒性降低.

关键词:
在无细胞转录的过程中.多药结合转录调节器.光学基因表达的方法可光活性染料的染料.转录规则 转录规则 转录规则

更多相关视频

Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds
09:44

Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds

Published on: October 15, 2019

12.1K
Two-Photon-Based Photoactivation in Live Zebrafish Embryos
09:10

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Published on: December 24, 2010

11.6K

相关实验视频

Last Updated: Jun 9, 2025

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
07:59

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Published on: March 22, 2018

7.6K
Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds
09:44

Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds

Published on: October 15, 2019

12.1K
Two-Photon-Based Photoactivation in Live Zebrafish Embryos
09:10

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Published on: December 24, 2010

11.6K

科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 视觉遗传学 视觉遗传学

背景情况:

  • 基因表达的光学调节提供了精确的时空控制.
  • 使用可光移动保护组的传统方法通常需要强烈的紫外线,并可能产生有毒的副产品.

研究的目的:

  • 开发一种新的,没有子组的系统,用于光介导的转录调节.
  • 用轻微可见光和可光激活染料实现转录激活.
  • 将该系统集成到用于体外和细胞应用的基因表达平台中.

主要方法:

  • 开发一种光介导的转录调节系统,将可光激活染料PaX560和转录调节器QacR结合起来.
  • 利用PaX560的无痕光转换产生一种基染料,该染料结合并调节QacR活性.
  • 将PaX560-QacR模块集成到T7RNA聚合酶表达系统中.

主要成果:

  • 在轻微可见光下通过PaX560介导的QacR调制来证明光激活的转录激活.
  • 实现了具有大动态范围的转录激活.
  • 启用了对光激活效应器状态的同时检测.
  • 在体外和活细胞内成功证明了光激活的转录.

结论:

  • 开发的系统为转录的光学控制提供了一种有效且不那么有毒的替代方案.
  • 这种新的方法允许精确,可见光诱导的基因表达操纵.
  • 该系统有可能在合成生物学和分子研究中获得先进的应用.