相关实验视频

Updated: Jun 9, 2025

Visualization and Quantification of High-Dimensional Cytometry Data using Cytofast and the Upstream Clustering Methods FlowSOM and Cytosplore

Visualization and Quantification of High-Dimensional Cytometry Data using Cytofast and the Upstream Clustering Methods FlowSOM and Cytosplore

Published on: December 12, 2019

使用FlowSOM优化稳定集群的参数:来自CyTOF的案例研究

Weiyang Tao¹, Anirban Sinha¹, Khadir Raddassi¹

¹Immunology Discovery Research, AbbVie Cambridge Research Center, Cambridge, MA, United States.

Frontiers in immunology

|October 24, 2024

概括

对FlowSOM包的关键修改改进了使用CyTOF的高维细胞表型化. 优化的参数使复杂的免疫细胞数据可靠地用于疾病研究的分析.

科学领域:

免疫学免疫学免疫学
计算生物学计算生物学
生物信息学是一种生物信息学.

背景情况:

使用CyTOF进行高维细胞表型鉴定对于了解健康和疾病至关重要.
分析复杂的CyTOF数据需要先进的计算方法,例如用于自动化门的机器学习.

研究的目的:

对基于机器学习的FlowSOM包进行批判性修改和优化,以实现可靠的CyTOF数据分析.
为了解决错误,并提高FlowSOM管道的大数据集的可扩展性.

主要方法:

修改了FlowSOM包,以修复现有的错误并提高可扩展性.
执行精确的参数优化,以评估其对集群结果的影响.
在大型外部免疫学数据集上验证了增强的FlowSOM管道.

主要成果:

关键修改和参数优化显著提高了CyTOF数据分析的可靠性.
确定了影响FlowSOM集群结果的关键参数.
证明了数据特定参数调整对于准确的免疫细胞群体定义的必要性.

结论:

修改后的FlowSOM管道提供了一种可靠的方法,用于使用CyTOF进行高维细胞表型化.
量身定制的参数优化对于在免疫疾病中询问免疫细胞群体至关重要.
这项工作增强了CyTOF在免疫学研究和疾病研究中的实用性.

关键词:

流量SOM 的流量.聚类集群是指聚类的聚类.通过 cyTOF,可以实现 cyTOF.一个高维数据集.参数优化的参数优化

更多相关视频

Characterization of Aquatic Biofilms with Flow Cytometry

Characterization of Aquatic Biofilms with Flow Cytometry

Published on: June 6, 2018

ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data

ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data

Published on: January 16, 2019

相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...