一种CH4驱动的离子云延伸方法使ICP-qMS能够进行多重单细胞分析.
Zhengxian Lv1, Xinli Yan1, Zhen Liu1
1Department of Chemistry & the MOE Key Lab of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
Chemistry (Weinheim an der Bergstrasse, Germany)
|October 24, 2024
概括
感应合等离子体四极质谱 (ICP-qMS) 现在可以分析单细胞. 甲气延长了离子云的持续时间,使细胞分类和异质性研究的蛋白质标记物的多重检测成为可能.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 质谱测量质量谱测量
背景情况:
- 感应合等离子四极质谱 (ICP-qMS) 是一种强大的工具,用于溶液中的元素和生物分子量化.
- 使用ICP-qMS分析完整的单个细胞是具有挑战性的,因为短暂的离子信号和仪器停留时间.
- 现有的ICP-qMS方法难以从悬浮中分散的单个细胞中获取多m/z信号.
研究的目的:
- 克服ICP-qMS在单细胞分析中的局限性.
- 为了使单个细胞上的蛋白质标记物的多重量化定量.
- 为了促进细胞分类和揭示细胞异质性使用ICP-qMS.
主要方法:
- 在碰撞反应电池 (CRC) 中利用甲 (CH4) 气体,延长单细胞离子云的持续时间.
- 实现了ICP- ((CH4-CRC) -qMS,用于从单个单元中获取多m/z信号.
- 应用了在单细胞上量化多个表型蛋白标记物的方法.
主要成果:
- 甲气把单细胞离子云的持续时间延长到7000微秒以上.
- 通过使用ICP-qMS从单个单元中成功获取多m/z信号.
- 能够量化多个蛋白质标记物,揭示单细胞异质性,并使亚型分类成为可能.
结论:
- 以CH4驱动的离子云拉伸方法克服了ICP-qMS单细胞分析中的一个主要瓶.
- 这种方法允许使用ICP-qMS对单细胞进行前所未有的多重分析.
- 为ICP-qMS在单细胞研究和诊断中的更广泛应用铺平了道路.
关键词:
乳腺癌细胞 乳腺癌细胞CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH5 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 CH4 是一个人可能是什么意思是什么意思是什么意思是什么意思碰撞-反应-细胞这就是ICP-qMS的意义.单细胞多重复合分析
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