相关实验视频

Updated: Jun 9, 2025

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

提高过敏原产品的质量控制:通过质谱测量来评估分子过敏原成分

Steffen Augustin¹, Thomas Eichhorn², Martin Wald¹

¹Allergopharma GmbH & Co, Reinbek, Germany.

|October 24, 2024

概括

这项研究证实,用于过敏测试和治疗的过敏原产品含有全面的过敏原,确保各批次的质量一致. 这种一致性对于有效的过敏管理至关重要.

科学领域:

过敏诊断和免疫治疗.
蛋白质组学和质谱学.
生物制药制造业的质量控制

背景情况:

个体敏感性概况需要全面的过敏原产品,以有效诊断和治疗过敏.
商用过敏原产品的过敏原成分可能会变化.
在诊断和治疗产品中确保完整的过敏原资料对于患者护理至关重要.

研究的目的:

评估皮肤刺伤测试 (SPT) 和过敏原免疫疗法 (AIT) 产品的定性过敏原成分.
为了评估这些过敏原产品的分批一致性.
验证一种高分辨率液态染色学合联质谱法 (LC-MS/MS) 方法用于过敏原分析.

主要方法:

使用一致的高分辨率LC-MS/MS方法进行定性过敏原分析.
分析的SPT和AIT产品来自常见的过敏原来源:草,树,花粉和室内灰尘.
调查了每个产品类型的三个不同的批次.

主要成果:

在分析的SPT和AIT产品中检测到所有主要,中级和大多数轻微过敏原.
在不同类型的产品中证明过敏原成分的完整性.
在过敏原含量方面证实了高程度的批量对批量一致性.

结论:

该研究提供了使用单一LC-MS/MS方法对SPT和AIT产品过敏原成分的第一个全面的定性数据.
经过验证的LC-MS/MS方法是可靠的,快速的,高吞吐量用于质量控制.
高分辨率LC-MS/MS适用于工艺开发,并确保准确反映自然过敏原来源的过敏原产品的可用性.

关键词:

过敏原的组成过敏原的组成过敏原免疫疗法免疫疗法过敏原产品产品过敏原产品室内灰尘虫室内灰尘虫质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量

更多相关视频

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

Author Spotlight: Advancing Biotherapeutic Mass Calculation by Introducing mAbScale, a Python-Based Desktop Application

Author Spotlight: Advancing Biotherapeutic Mass Calculation by Introducing mAbScale, a Python-Based Desktop Application

Published on: June 16, 2023

相关概念视频

Mass Spectrometry: Overview

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

High-Resolution Mass Spectrometry (HRMS)

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...

Mass Spectrometers

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:

Mass Spectrum: Interpretation

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a low-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.
To...