叶子和WAPO1的自然等位基因相互作用,调节小麦中每的尖端数量
Junli Zhang1, Germán F Burguener1,2, Francine Paraiso1,2
1Department of Plant Sciences, University of California, Davis, CA, 95616, USA.
概括
叶子 (LFY) 和APO1 (WAPO1) 的小麦正基因的天然小麦等位基因的特定组合,最大限度地增加了每个尖端的尖端数量. 这些发现为具有更高谷物产量潜力的育种小麦提供了洞察力.
科学领域:
- 植物遗传学和育种.
- 农作物科学 农作物科学
- 分子生物学分子生物学
背景情况:
- 每个尖的尖数 (SNS) 是小麦产量的关键组成部分.
- 在APO1 (WAPO1) 的叶子 (LFY) 或小麦 (WAPO1) 的功能丧失突变通过影响尖干干干形成来降低SNS.
- 之前已经证明,一种天然的WAPO1变体 (C47F) 增加了SNS.
研究的目的:
- 为了识别与小麦中每 (SNS) 尖数量的差异相关的自然叶子 (LFY) 变异.
- 调查自然LFY和WAPO1变体之间的相互作用,以研究它们对SNS的影响.
- 提出最佳的等位基因组合,以提高小麦产量潜力.
主要方法:
- 在嵌套的协会映射群体中搜索自然LFY变体.
- 证实了LFY-B多态R80S与SNS差异的关联,使用了大量的映射群体.
- 进行了单双类型分析,并探索了双亲种群中的LFY-WAPO1变体相互作用.
主要成果:
- 鉴定了LFY-B变种,特别是P34L和R80S在SNS上的显著影响.
- 发现这些LFY-B等位基因在小麦化和繁殖过程中被丰富,这表明了积极的选择.
- 发现LFY和WAPO1变体之间的显著相互作用,其中LFY-B等位基因 (34L,80S) 仅在WAPO-A1-47F背景中增强了SNS.
结论:
- 在小麦中,LFY和WAPO1天然基因的特定组合最大限度地增加了每尖端的尖端数 (SNS).
- 代基组合WAPO-A1-47F/LFY-B 34L 80S可以在小麦育种计划中使用.
- 这种组合有可能最大限度地提高SNS,并增加小麦的谷物产量潜力.
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