开发一种工程 IL-36γ 过分分泌的 Lactococcus lactis,改善肠道环境
Masahiro Yoda1, Shogo Takase1, Kaho Suzuki1
1Department of Biomolecular Innovation, Institute for Biomedical Sciences, Shinshu University, Nagano, 399-4598, Japan.
World journal of microbiology & biotechnology
|October 24, 2024
概括
改造的乳球球菌 (Lactococcus lactis) 细菌向肠道输送介素-36 (IL-36γ),增强粘膜免疫力,促进有益的肠道细菌. 这种益生菌方法对预防肠道疾病充满希望.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 胃肠病学 胃肠病学
背景情况:
- 介素-36 (IL-36) 是一种对粘膜免疫至关重要的促炎细胞因子.
- 肠道疾病可能是由受损的粘膜防御引起的.
- 针对性地将IL-36输送到肠道是一个潜在的治疗策略.
研究的目的:
- 为了设计乳球菌为生产和输送重组小鼠IL-36γ (rmIL-36γ) 到肠道.
- 评估这种工程益生菌在调节肠道环境和免疫反应方面的有效性.
主要方法:
- 在尼诱导下对乳球菌乳糖进行基因工程,以表达rmIL-36γ.
- 通过口服给小鼠注射工程细菌 (NZ-IL36γ).
- 使用PCR和ELISA确认细菌殖民和rmIL-36γ分泌.
- 对肠道微生物群组成和结肠中的Muc2基因表达的分析.
主要成果:
- 工程NZ-IL36γ菌株成功地在小鼠肠道中产生和分泌rmIL-36γ.
- 服用NZ-IL36γ导致了有益的肠道细菌 (例如,乙化因子,Roseburia) 的丰富性增加.
- 在结肠中观察到Muc2表达的升高,表明增强了屏障功能.
结论:
- 乳球菌是一种可行的载体,可以将rmIL-36γ输送到肠道粘膜.
- 这种益生菌策略有效调节肠道微生物群,增强肠道屏障功能.
- NZ-IL36γ具有作为改善肠道健康和预防肠道疾病的新疗法工具的潜力.
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