在耐里芬胺耐药的 Mycobacterium Tuberculosis 隔离物中,II型毒素-抗毒素系统和毒素的分布
Maryam Shafipour1, Abdolmajid Mohammadzadeh1, Pezhman Mahmoodi1
1Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran.
PloS one
|October 24, 2024
概括
马兹F3基因的突变与Mycobacterium结核病菌的里法胺耐药性有关,特别是在2系. 这一发现突显了毒素-抗毒素系统在药物耐药性的作用.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 二类毒素-抗毒素 (TA) 系统,包括mazEF3,vapBC3和relJK,与细菌抗生素耐药性和耐受性有关.
- 这些特定的TA系统在各种细菌病原体的耐药性发展中发挥着至关重要的作用.
研究的目的:
- 在敏感和耐药的Mycobacterium结核病分离物中研究mazEF3,vapBC3和relJKII型毒素-抗毒素系统中的突变.
- 为了将这些基因突变与里法抗性和不同M.结核病血统相关联.
主要方法:
- 基因组DNA从153个M.结核病分离物中提取 (32个耐利,121个耐利敏感),这些分离物是在伊朗收集的.
- 使用放大阻燃突变系统-聚合酶链反应 (ARMS-PCR) 进行了谱系类型化.
- 在rpoB基因的突变被分析为多重扩大特定等位基因-聚合酶链反应 (MAS-PCR),在mazEF3,relJK和vapBC3的突变被通过PCR测序识别.
主要成果:
- 血统2在研究的M.结核病分离物中表现出对利法素耐药性的最高患病率.
- 对rpoB531的突变是观察到的最常见的基因变异在抗利法的分离物中.
- 在mazF3基因中,核酸195 (codon 65) 的特定突变导致了三氨酸替代异黄氨酸的氨基酸替代,在所有抗利法的隔离物中始终发现.
结论:
- 在mazF3基因中发现的突变是Mycobacterium tuberculosis中与里法抗性相关的显著标志物.
- 毒素-抗毒素系统,特别是mazEF3,是M.结核病中耐药性的出现和发展的关键组成部分.
- 用更大的样本大小进行进一步的研究可能会导致用于检测耐药M.结核病菌株的新型诊断方法.
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