对某些葡萄藤病原体进行内菌的分子鉴定和抗菌潜力
Lava H Nashat1, Raed A Haleem2, Shayma H Ali1
1Department of Biology, College of Sciences, University of Duhok, Duhok, Duhok Province, Kurdistan Region, Iraq.
PloS one
|October 24, 2024
概括
在伊拉克葡萄藤中分离出的内菌显示出对葡萄藤疾病的生物控制剂的潜力. 这项研究强调了它们在葡萄园可持续管理中的作用,并提供了新的害虫控制策略.
科学领域:
- 植物病理学 植物病理学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 内生菌在植物组织中共生,提供诸如增强营养获取和耐压力等好处.
- 这些真菌产生生物活性化合物,在农业中具有潜在的应用.
- 葡萄树干疾病和腐烂真菌显著影响作物产量和健康.
研究的目的:
- 为了隔离和识别来自伊拉克葡萄酒中的内菌.
- 评估这些内植物对关键葡萄藤病原体的生物控制潜力.
- 建立伊拉克葡萄树上特定内菌的第一个记录.
主要方法:
- 来自伊拉克杜霍克省四个地点的600个葡萄树样本的采集 (2021-2022).
- 隔离和分子识别 (ITS和SSU测序) 的内菌.
- 在实验室中使用双培养和食物中毒方法对病原体的生物控制疗效进行评估,这些病原体包括 Ilyonectria destructans, Botrytis cinerea 和 Neoscytalidium dimidiatum.
主要成果:
- 隔离了12种内生菌种,其中5种 (Penicillium radiatolobatum,Botrysphaeria dothidea,Fusarium ruscicola,Fusarium venenatum和Paecilomyces maximus) 是伊拉克葡萄树的新纪录.
- 最大的Paecilomyces表现出一个70.83%的抑制率对Ilyonectria destructans.
- 亚斯伯吉illus flavipes,P.maximus,Aspergillus terreus,Mucor racemosus和P.maximus对I. destructans,Botrytis cinerea和Neoscytalidium dimidiatum进行了显著的抑制.
结论:
- 来自伊拉克葡萄树 (Vitis vinifera) 的内菌具有重要的生物控制能力,可以对抗主要的葡萄藤疾病.
- 这些发现支持使用内菌作为葡萄园综合性害虫防治的可持续策略.
- 进一步研究和应用这些真菌内植物可以提高葡萄藤的健康和生产力.
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