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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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相关实验视频

Updated: Jun 9, 2025

Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells

Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells

Published on: May 23, 2025

MALDI MSI协议用于空间自下而上的蛋白质组学在单细胞分辨率.

Andrej Grgic¹, Eva Cuypers¹, Ludwig J Dubois²

¹The Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry (IMS), Maastricht University, 6229 ER Maastricht, The Netherlands.

Journal of proteome research

|October 24, 2024

概括

此摘要是机器生成的。

这项研究引入了一种用于矩阵辅助激光消耗/电离质谱成像 (MALDI-MSI) 分析单细胞的新协议. 这种方法实现了自下而上的空间蛋白质组学的高空间分辨率,使单细胞蛋白质的详细分析成为可能.

关键词:

马尔迪马尔迪马尔迪马尔迪在MSI中,它是MSI.高空间分辨率的高空间分辨率.质谱仪成像成像质谱仪成像成像蛋白质组学蛋白质组学一个单细胞的单细胞.

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Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

相关实验视频

Last Updated: Jun 9, 2025

Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells

Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells

Published on: May 23, 2025

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

科学领域:

蛋白质组学是指蛋白质组学.
质谱仪成像成像质谱仪成像
细胞生物学细胞生物学

背景情况:

矩阵辅助激光脱/电离质谱成像 (MALDI-MSI) 已经发展为和蛋白质的空间映射.
现有的自下而上的蛋白质组学协议在空间分辨率和单细胞分析样本准备方面面临挑战.

研究的目的:

开发和优化2D培养单细胞MALDI-MSI的协议.
为了实现底部向上空间蛋白质组学的单细胞分辨率.

主要方法:

使用CHCA升华和酸单基 (AmP) 浸入优化样品制备.
在ITO幻灯片上培养的单细胞上进行MALDI-MSI测量.
整合MALDI-MSI数据与来自细胞颗粒的液体染色体-双重质谱 (LC-MS/MS) 数据.

主要成果:

实现了400nm左右的矩阵晶体大小,从而实现了高空间分辨率.
从单个MDA-MB-231乳腺癌细胞中检测到89个类似的特征.
通过结合MALDI-MSI和LC-MS/MS数据,从17种蛋白质中鉴定出24种,包括actin和vimentin.

结论:

开发的协议使单细胞的高通量MALDI-MSI成为可能.
这种工作流在单细胞水平上显著推进了自下而上的空间蛋白质组学.
该方法提供了对单个细胞内的蛋白质分布的见解.