普特雷辛补充剂提高了体外生产的牛胚胎的发育能力
Kubra Karakas Alkan1, Fatma Satilmis1, Gonca Sonmez2
1Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye.
Theriogenology
|October 24, 2024
概括
补充牛胚胎培养与普特瑞辛,一种抗亡和抗氧化化合物,显著改善了胚胎的发育和质量. 这项研究发现,在治疗普特时,更高的胚胎细胞发育率,细胞数量增加,亡和反应性氧物种 (ROS) 减少.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 生物化学 生物化学
背景情况:
- 试管胚胎生产 (IVEP) 对于牛养殖和研究至关重要.
- 优化培养基对于改善胚胎发育和质量至关重要.
- 聚胺之一的普特雷斯具有抗亡和抗氧化特性.
研究的目的:
- 调查普特瑞辛补充剂在体外培养过程中对牛胚胎发育和质量的影响.
- 评估普特瑞辛对胚胎细胞形成速度,细胞数量,细胞亡和活性氧物种 (ROS) 水平的影响.
主要方法:
- 荷尔斯坦牛的卵细胞在体外成熟并受精.
- 据推测,在含有或不含0.5毫米普特雷辛的介质中培养了假定的阴茎.
- 分析了母细胞细胞的发育,细胞计数 (ICM,TE,TCC),TUNEL测定细胞亡,ROS水平和基因表达.
主要成果:
- 普特雷辛补充剂显著增加了囊胞形成率 (44.37%与32.97%相比).
- 来自普特雷辛组的胚胎囊显示出更高的内细胞质量 (ICM),热体皮 (TE) 和总细胞计数 (TCC).
- 普特瑞辛减少了亡细胞,降低了亡指数,降低了ROS水平,同时对抗氧化剂和抗亡基因表达 (SOD,GPX4,CAT,BCL2,NANOG,GATA3) 进行上调调节,并对亲亡BAX进行下调调节.
结论:
- 普特雷辛补充剂增强了早期胚胎发育,并提高了体外生产的牛胚胎的质量.
- 这些有益作用归因于普特雷辛的抗氧化和抗丧性质,导致细胞应激减少和改善发育潜力.
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