相关实验视频

Updated: Jun 9, 2025

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

Published on: April 3, 2018

在人牙纤维细胞中模仿和体外验证慢性炎症

Anne Eriksson Agger¹, Athina Samara¹, Tianxiang Geng²

¹Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway; FUTURE, Center for Functional Tissue Reconstruction, University of Oslo, Oslo, Norway.

Archives of oral biology

|October 24, 2024

概括

研究人员开发了一种体外模型来模仿人类牙炎症. 这个模型使用减少的血清,大肠杆菌脂多糖化物 (LPS) 和介素-1β (IL-1β) 来复制体内细胞因子和化学因子的概况.

科学领域:

牙周研究牙周研究
在体外建模模型.
免疫学免疫学免疫学

背景情况:

牙炎症涉及复杂的细胞因子和化学因子信号传递.
准确的体外模型对于研究牙周病机制至关重要.
现有的模型可能无法完全复制体内炎症概况.

研究的目的:

识别和验证模仿人类炎症牙的体外条件.
建立一个可靠的模型来研究翻译性细胞因子和化学因子的概况.
了解特定刺激对纤维细胞反应的影响.

主要方法:

主要的人类牙纤维细胞 (HFIB-G) 被培养.
细胞接受了来自大肠杆菌和P. gingivalis的不同度的脂聚糖化物 (LPS) 和复合性互白素-1β (IL-1β) 的治疗.
使用Luminex测量细胞因子/化学因子水平,并在血清饥饿条件下通过微阵列分析基因表达.

主要成果:

大肠杆菌LPS和IL-1β单独增加了IL-6和MCP-1的分泌.
大肠杆菌LPS,IL-1β和血清饥饿的组合显著增加了IL-6,TNFα和其他炎症因素.
基因表达分析显示增强了T辅助细胞信号传递和矩阵金属蛋白酶活性.

结论:

血清减少与大肠杆菌LPS和IL-1β相结合,在体内有效模仿慢性炎症.
这种体外模型准确地复制了在炎症的牙组织中发现的基因表达和分泌的特征.
经过验证的模型为牙周研究提供了一个强大的平台.

关键词:

人类牙纤维细胞.免疫学免疫学免疫学炎症炎症是一种炎症.互乐金1ββ的存在脂聚糖类糖化物脂聚糖类糖化物牙周病是指牙周病的发生.

更多相关视频

Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network

Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network

Published on: February 16, 2016

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

Published on: August 8, 2014

相关概念视频

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...