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通过调节RNF144A-FTO轴,FOXF2通过降低M2 TAMs来抑制食道状细胞癌

Tianci Han¹, Wei Tong¹, Junwei Xie¹

¹Department of Thoracic Surgery, Cancer Hospital of Dalian University of Technology, Shenyang 110042, China; Department of Thoracic Surgery, Liaoning Cancer Hospital & Institute, Shenyang 110042, China.

International immunopharmacology

|October 24, 2024

概括

叉头盒F2 (FOXF2) 在食道状细胞癌 (ESCC) 中下调. 抑制FOXF2通过向FTO/RNF144A轴来抑制ESCC细胞的增殖和M2巨的偏离.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

食道状细胞癌 (ESCC) 具有高侵袭性和低生存率.
瘤相关巨细胞 (TAMs) 显著影响瘤进展,转移和免疫抑制.
叉头盒F2 (FOXF2),FOX家族的成员,在ESCC下调,其确切的作用和机制尚不清楚.

研究的目的:

研究FOXF2在ESCC进展中的作用及其与TAMs的相互作用.
阐明FOXF2在ESCC中的作用的基础分子机制.

主要方法:

在ESCC中的差异基因表达分析.
在ESCC细胞中,FOXF2的过度表达和沉默.
评估ESCC细胞增殖和TAM M2极化.
调查FOXF2,RNF144A和FTO之间的监管关系.

主要成果:

在ESCC组织中发现FOXF2的下调.
过度表达FOXF2抑制了ESCC细胞增殖和M2 TAM两极分化,而对FOXF2进行沉默则产生了相反的效果.
确定FOXF2可以促进RNF144A转录,从而导致FTO (m6A脱甲基酶) 的无化和降解.
FTO过度表达抵消了FOXF2对TAM极化的影响.

结论:

通过抑制瘤细胞增殖和M2 TAM极化,FOXF2在缓解ESCC方面发挥着至关重要的作用.
该机制涉及FOXF2诱导的RNF144A转录,它针对FTO进行降解.
FOXF2/RNF144A/FTO轴代表了ESCC治疗的潜在治疗目标.

关键词:

在 ESCC ESCC 的位置上.这里是 FOT FOT.这是FOXF2F2 在 RNF144A 中.这就是TAMs.

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