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开发一种ELISA来有效确定复合狂犬病人类单克隆抗体的有效性

Ambika Divase¹, Sambhaji Pisal², Manjusha Dake³

¹Serum Institute of India Pvt. Ltd., Hadapsar, Pune 411028, Maharashtra, India; Dr. D.Y. Patil Biotechnology and Bioinformatics Institute, Dr. D.Y. Patil Vidyapeeth, Pune 411033, Maharashtra, India.

Journal of immunological methods

|October 24, 2024

概括

一个新的ELISA测试提供了一种更快,更容易获得的方法来量化狂犬病单克隆抗体效力. 这种具有成本效益的测试取代了传统的RFFIT,简化了资源有限的环境中的狂犬病诊断.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
生物技术是生物技术.

背景情况:

快速光聚焦抑制试验 (RFFIT) 是用于狂犬病单克隆抗体功效的标准,但资源密集.
世卫组织鼓励使用替代方法来克服RFFIT的局限性,包括时间,生物安全和专业人员要求.

研究的目的:

开发和验证一种体外ELISA作为RFFIT的优质替代品,用于量化狂犬病单克隆抗体效力.

主要方法:

使用非活性狂犬病病毒疫苗 (Rabivax-S) 开发了一种间接ELISA.
试验验证包括准确性,精度 (20%内CV),线性 (r2>0.98),LLOQ (0.3μg/mL) 和特异性测试.

主要成果:

艾丽莎检测结果显示,对于狂犬病单克隆抗体量化,其灵敏度,精度,稳定性和准确性都很高.
验证证实了出色的线性和低量化极限,表明了可靠的测定.

结论:

开发的ELISA是一种精确,准确和敏感的方法,用于量化狂犬病单克隆抗体功效.
这种测试具有成本效益,易于执行,周转时间短,不需要BSL-2设施或活病毒,因此适用于低资源环境.

关键词:

狂犬病单克隆抗体狂犬病单克隆抗体间接的艾丽莎艾丽莎间接的艾丽莎实力测试实力测试实力测试实力测试狂犬病病毒病毒的病毒.

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相关概念视频

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.