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HSF1/HSP25系统保护线粒体功能免受热应激,并协助MA-10莱迪格细胞中的类固醇生成

Shintaro Oka¹, Ryosuke Takii², Mitsuaki Fujimoto²

¹Department of Urology, Yamaguchi University School of Medicine, Ube, Japan; Department of Biochemistry and Molecular Biology, Yamaguchi University School of Medicine, Ube, Japan.

Molecular and cellular endocrinology

|October 24, 2024

概括

热冲击转录因子1 (HSF1) 稳定了类固醇性急性调节蛋白 (StAR) 并维持了线粒体功能. HSF1/HSP25通路对于在热应激下保护类固醇生成至关重要.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
内分泌学在内分泌学.

背景情况:

热冲击反应涉及热冲击蛋白 (HSP),并由热冲击转录因子1 (HSF1) 调节.
在压力下,HSF1对于维持蛋白质平衡和细胞功能至关重要.
HSF1在类固醇生成中的作用,特别是在莱迪格细胞中,仍然不完全理解.

研究的目的:

为了研究HSF1在热应激下对类固醇生成的影响.
阐明HSF1途径在调节线粒体功能和类固醇性急性调节蛋白 (StAR) 稳定性方面的作用.

主要方法:

通过CRISPR-Cas9基因组编辑产生HSF1-淘汰赛MA-10小鼠莱迪格瘤细胞.
对STAR蛋白和mRNA水平,HSP表达,线粒体膜潜力 (MMP) 和在热应激下ATP合成的分析.
使用碳烯化物3-甲 (CCCP) 的MMP的药理学干扰和通过敲击和过度表达评估HSP25的作用.

主要成果:

在热应激下,HSF1缺乏导致Star蛋白水平降低和线粒体功能受损 (减少MMP和ATP合成).
HSF1调节HSP110,HSP70和HSP25的表达.
HSP25起着保护作用,其敲击降低了MMP和STAR水平,而其在HSF1敲击细胞中的过度表达恢复了STAR水平.

结论:

在热应激期间,HSF1/HSP25通路对于维持线粒体功能和Star蛋白稳定性至关重要.
这一途径对于保护莱迪格细胞中的类固醇生成至关重要.
通过HSF1调节HSP25,是细胞适应热应激的关键机制.

关键词:

热冲击蛋白 25 热冲击蛋白 25 热冲击蛋白热冲击转录因子1热冲击转录因子1 热应激反应的热应激反应莱迪格细胞是莱迪格细胞.类固醇的急性调节蛋白质.

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