两种GacS调节的脂和三型分泌系统都会导致Pseudomonas cichorii诱导的生菜和西瓜的亡
Chien-Jui Huang1, Ellen Pauwelyn2, Marc Ongena3
1Department of Plants and Crops, Laboratory of Phytopathology, Ghent University, Coupure Links, 653, 9000 Ghent, Belgium; Department of Plant Medicine, National Chiayi University, No. 300, Syuefu Rd., 600355, Chiayi, Taiwan, Republic of China.
Research in microbiology
|October 24, 2024
概括
伪虫使用脂和三型分泌系统 (T3SS) 来引起疾病. 毒性因子取决于宿主植物,GacS调节的脂和T3SS在香草和生菜感染中起着关键作用.
科学领域:
- 植物病理学 植物病理学
- 微生物病原体的产生
- 细菌的毒性因素 细菌的毒性因素
背景情况:
- 伪菌 (Pseudomonas cichorii SF1-54) 导致生菜中肋骨腐烂,产生对毒性至关重要的脂 (cichofactins,cichopeptins).
- GacS/GacA两组系统调节了伪月球中的脂产量.
- 第三类分泌系统 (T3SS) 在P. cichorii与植物相互作用中的作用尚不清楚.
研究的目的:
- 研究GacS调节的脂和T3SS在P. cichorii SF1-54病原性中的作用.
- 确定这些因素对香草和生菜的毒性贡献.
主要方法:
- 在P. cichorii SF1-54.4中的hrpL (T3SS调节器) 和 gacS (脂调节器) 中构建突变.
- 在野生型和突变菌株中评估脂产量.
- 评估了和生菜植物的毒性和殖民性.
主要成果:
- 这种hrpL突变表现出与野生类型相似的脂水平和毒性.
- 这种 gacS 突变已经损害了脂的产生,并在两个宿主身上减少了毒性.
- 这种 gacS hrpL 双重突变在香草上缺乏脂,毒性和殖民,但在生菜上保留了弱毒性.
结论:
- GacS调节的脂和T3SS以宿主依赖的方式为P. cichorii的毒性做出贡献.
- 脂对花感染至关重要,而这两种因素都与生菜感染有关.
- 宿主植物之间,P. cichorii的致病机制显著不同.
关键词:
奇科法克因 (Cichofactin) 是一种可靠的药物.齐科佩普丁是一种可怕的药物.循环性脂类类.这就是" Gac regulon regulon " 的意思.乳树 (Lactuca sativa) L. var. 的一种植物. 一个名为capita capitata的公司.在中腹部腐烂.更多相关视频
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