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皮塞坦诺尔通过调节Keap1-Nrf2/ARE信号通路来保护精子免受冷保存损伤
Lijie Fu1, Chao Wang2, Wenfu Li2
1Department of Urological Surgery No.2, First Affiliated Hospital of Kunming Medical University, No. 295 Xichang Road, Kunming, 650032, Yunnan Province, China. kmmedflj@sina.com.
Reproductive sciences (Thousand Oaks, Calif.)
|October 24, 2024
概括
在冷保存期间,Piceatannol (PIC) 通过通过Keap1-Nrf2通路减少氧化应激来保护精子. 这提高了冷解后的精子运动性和活力,为精子保存提供了一种新的方法.
科学领域:
- 生殖生物学 生殖生物学
- 氧化压力研究研究 氧化压力研究
- 冰冷保存科学 冰冷保存科学
背景情况:
- 精子的冷保存可能导致氧化损伤,降低生命力.
- 皮塞阿坦诺 (Piceatannol,简称PIC) 是一种具有抗氧化功能的多.
- 了解PIC在精子冷保存中的机制对于改善生育结果至关重要.
研究的目的:
- 调查Piceatannol (PIC) 在冷保存期间对精子的氧化损伤的保护机制.
- 阐明PIC在冷解精子中调节活性氧物种 (ROS) 和抗氧化途径中的作用.
主要方法:
- 在冷解过程中,精液样本用不同度的PIC进行了处理.
- 评估了精子运动性 (CASA),血膜完整性 (HOS测试),ROS,SOD,T-AOC和MDA水平.
- 使用qRT-PCR分析了Keap1,Nrf2,GCLC,GCLM和HMOX1的基因表达.
主要成果:
- PIC (5.0μmol/L) 显著改善了精子的运动性,活力和血膜完整性.
- PIC治疗降低了ROS水平,调节了抗氧化酶活性.
- 在PIC上调的Nrf2通路组件 (Nrf2,GCLC,GCLM,HMOX1) 和下调的Keap1表达.
- 抑制Nrf2消除了PIC的保护作用,增加了ROS.
结论:
- 皮塞坦诺尔通过Keap1-Nrf2/ARE通路保护精子免受冷诱导的氧化应激.
- 通过调节ROS水平和激活抗氧化防御,PIC提高了解后的精子活力.
- 这项研究将Piceatannol作为优化精液冷保存协议的有希望的代理商.
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