保持在不同温度和时间间隔的心脏组织中的DNA甲基化稳定性
Brando Poggiali1, Mikkel Eriksen Dupont2, Stine Bøttcher Jacobsen2
1Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. brando.poggiali@sund.ku.dk.
Scientific reports
|October 25, 2024
概括
心脏组织中的DNA甲基化 (DNAm) 在4°C下至少稳定28天,在22°C下稳定1天,这使得在心血管疾病研究中可靠的表观遗传分析成为可能,即使样本存储次优.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 心血管研究研究心血管研究
- 基因组学就是基因组学.
背景情况:
- 基因甲基化 (DNAm) 分析对于理解心血管疾病 (CVD) 是至关重要的.
- 在手术期间收集的心脏活检可能因资源限制而面临存储延迟.
- 在非最佳条件下评估DNAm稳定性对于实际应用至关重要.
研究的目的:
- 为了研究在不同温度和持续时间储存的人类心脏组织中全基因组DNAm的稳定性.
- 为了确定延迟储存对DNAm完整性的影响,为未来的表观遗传学研究.
主要方法:
- 人类心脏组织在4°C和22°C下存储长达28天.
- 通过Illumina Infinium MethylationEPIC v1.0 BeadChip Array进行了DNA甲基化分析.
- 评估了相关性和差异甲基化位置 (DMP).
主要成果:
- 在立即分析和存储样本之间观察到高相关性 (R2 > 0.992).
- 在4°C持续28天或22°C持续1天时,没有显著的DNAm变化发生.
- 在7,14和28天后,在22°C出现了显著的DMP,观察到数千个变化.
结论:
- 心脏组织DNAm在4°C下长时间 (28天) 非常稳定.
- 在室温下短期储存 (22°C1天) 也可以保持DNAm的完整性.
- 这些发现支持从心脏样本中进行mDNA分析的可行性,这些心脏样本在手术后存储在亚最佳状态.
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