相关实验视频

Updated: Jun 9, 2025

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

Published on: June 26, 2019

大脑血管和腹膜之间的差异性硬度通过血管选择促进了转移性透

Marina Uroz^1,2,3, Amy E Stoddard^1,2,3,4, Bryan P Sutherland^1,2,3

¹Department of Biomedical Engineering, Boston University, Boston, MA, USA.

Nature cell biology

|October 25, 2024

概括

癌细胞通过选择血管来入侵大脑,这是由血管和大脑组织之间的机械差异驱动的. 这个过程依赖于血管的刚性和周围组织的软度,揭示了大脑转移的一个关键机制.

科学领域:

生物物理学的生物物理.
癌症生物学癌症生物学
细胞迁移细胞迁移

背景情况:

大脑转移涉及癌细胞使用血管迁移 (血管合作).
规范船舶合作的确切机制在很大程度上是未知的.
了解这些机制对于开发有针对性的疗法至关重要.

研究的目的:

阐明调节癌细胞沿脑血管迁移的生物力学因素.
为了研究组织硬度在脑转移期间血管选择中的作用.

主要方法:

使用了ex vivo脑部切片和一个器官类型的体外模型.
采用先进的成像分析来追踪癌细胞的运动.
研究了细胞外矩阵和焦点粘附动态的影响.

主要成果:

沿大脑血管系统的癌细胞入侵是由血管和腹膜之间的刚性差异驱动的.
细胞沿着血管的基底表面迁移,粘附于底层膜.
血管合作通过血管硬度 (依赖塔林) 和组织软度得到增强.

结论:

组织机械异质性决定了癌细胞在大脑转移期间的迁移.
一个涉及焦点粘附和细胞外基质的机械感知机制指导血管合作.
这些发现提供了关于癌症侵袭和转移的见解,可能为治疗策略提供信息.

更多相关视频

Modeling Brain Metastasis Via Tail-Vein Injection of Inflammatory Breast Cancer Cells

Modeling Brain Metastasis Via Tail-Vein Injection of Inflammatory Breast Cancer Cells

Published on: February 4, 2021

Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography

Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography

Published on: August 16, 2020

相关概念视频

Metastasis

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...

The Blood-brain Barrier

The Blood-brain Barrier

Mechanism of Angiogenesis

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...