通过激活细胞的抗体测量记忆B细胞反应
Caroline Rockstroh1, Katja Hintz1, Judith Kannenberg1
1Institute for Medical Microbiology and Virology, University Hospital and Medical Faculty, University of Leipzig, 04103 Leipzig, Germany.
通过比较记忆B细胞 (MBC) 测试,MBC-ELISA和MBC-ELISpot测试都显示出相似的灵敏度和强烈的相关性,使它们在测量免疫反应方面可以互换. MBC-ELISA提供了扩展分析的灵活性.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
背景情况:
- 记忆B细胞 (MBCs) 在感染或接种疫苗后对长期免疫至关重要.
- 评估MBC反应的强度对于了解免疫保护至关重要.
- 为了量化MBC反应,存在各种测试,但它们的可比性并不总是清楚.
研究的目的:
- 为了比较MBC酶链接免疫吸收试验 (MBC-ELISA) 和MBC酶链接免疫位点 (MBC-ELISpot) 试验的性能.
- 为了确定MBC-ELISpot和MBC-ELISA的检测下限.
- 评估MBC-ELISA和MBC-ELISpot测量在单个样本和人群中的相关性.
主要方法:
- 一种MBC-ELISpot试验被用于在体外计算来自MBC的抗体分泌细胞 (MASCs).
- 两种版本的MBC-ELISA被用于测量MASCs分泌的抗体度.
- 统计分析了较低的检测极限和测试之间的相关性.
主要成果:
- 无论是MBC-ELISpot还是MBC-ELISA,都显示出可比较的检测下限.
- 在单个样本中的抗体度和MASC计数之间观察到强烈的相关性 (R = 0.83-0.87).
- 抗原特异性抗体的比例与抗原特异性MASCs的比例相关得很好 (R = 0.74-0.82).
结论:
- 由于类似的灵敏度和强烈的测量相关性,MBC-ELISA和MBC-ELISpot可以互换.
- MBC-ELISA提供了优势,包括能够在时间上分割,重复和扩展抗体测量的能力.
- 这些发现扩大了对MBC响应评估的可能性.
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