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动态微光连贯断层扫描使哺乳动物尾虫的结构和代谢成像成为可能

Hinnerk Schulz-Hildebrandt¹, Svetolik Spasic², Fang Hou¹

¹Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States.

Frontiers in molecular neuroscience

|October 25, 2024

概括

动态微光连贯断层扫描 (DμOCT) 可视化耳细胞代谢和活力. 这种先进的成像工具在细胞层面提供了对神经传感器听力损失 (SNHL) 的高分辨率洞察,有助于诊断和治疗评估.

科学领域:

耳鼻喉科耳鼻喉科耳鼻喉科
生物医学工程生物医学工程
细胞成像细胞成像

背景情况:

感官神经听力损失 (SNHL) 源于对耳毛细胞和神经元的损伤.
高分辨率的耳细胞成像对于诊断SNHL和评估治疗疗效至关重要.
耳的解剖学约束在历史上限制了体内细胞成像.

研究的目的:

推进微光相干断层扫描 (μOCT) 以同时,共同定位的耳细胞活力和代谢活动的成像.
开发动态μOCT (DμOCT) 用于可视化细胞内运动,表明细胞功能.
证明DμOCT在小鼠尾细胞中解决细胞和代谢特征的能力.

主要方法:

开发了一个定制的DμOCT系统.
获得的顺序μOCT图像和计算的像素智能的强度波动频率指标.
利用依赖ATP的细胞内器官运动和细胞骨聚合作为代谢活动的指标.
用DμOCT通过角耳管切除术将DμOCT应用于刚切除的成年老鼠耳.

主要成果:

在Corti的器官中实现了解像度高的解像度解剖学和代谢特征的成像.
证明了DμOCT能够捕获耳细胞中快速代谢变化的能力.
展示了只需6就可以以足够的细节重建DμOCT图像,以辨别单个细胞及其代谢状态.
成功可视化了细胞内运动,ATP依赖器官运动和细胞骨聚合.

结论:

DμOCT提供了一种评估耳细胞活力和代谢状态的新方法.
这项技术可实现高分辨率,细胞水平的耳功能成像.
结果支持未来开发用于SNHL临床诊断的DμOCT探针.

关键词:

甲状腺 (cochlea) 是一种头发细胞是指毛细胞.代谢成像 - - 代谢成像微光连贯性断层扫描微光连贯性断层扫描科蒂的器官科蒂的器官感应神经神经听力损失

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The Cochlea

The Cochlea

The cochlea is a coiled structure in the inner ear that contains hair cells—the sensory receptors of the auditory system. Sound waves are transmitted to the cochlea by small bones attached to the eardrum called the ossicles, which vibrate the oval window that leads to the inner ear. This causes fluid in the chambers of the cochlea to move, vibrating the basilar membrane.

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...