活细胞光超分辨率显微镜中的线粒体的光白和光毒性
Chia-Hung Lee1, Douglas C Wallace2, Peter J Burke3
1Department of Biomedical Engineering, University of California, Irvine, CA, 92697, United States.
Mitochondrial communications
|October 25, 2024
概括
超分辨率显微镜中的光毒性会损害线粒体,改变它们的形状和膜潜力. 10-N-nonyl acridine orange (NAO) 证明比其他染料更具有光毒性,提供了一种评估染料-照明组合的方法.
科学领域:
- 细胞生物学 细胞生物学
- 显微镜的使用方法
背景情况:
- 光漂白和光毒性是超分辨率显微镜中的重大挑战,影响细胞活力和数据完整性.
- 评估光毒性是复杂的,因为有多种因素.
研究的目的:
- 在超分辨率成像过程中实证评估不同光染料在线粒体上的光毒性.
- 研究光漂白和光毒性对线粒体结构和功能的影响.
主要方法:
- 使用了Airyscan超高分辨率显微镜.
- 检查了米托拉克绿 (MTG),10-N-nonyl acridine色 (NAO) 和四甲基胺,乙烯 (TMRE) 对线粒体的影响.
- 评估了线粒体形状,数和膜潜力的变化.
主要成果:
- 光毒性诱导线粒体的形状从管状变为球状和缩小的晶状体.
- 光毒性显著影响了线粒体膜潜力.
- 与MTG和TMRE相比,NAO对线粒体的光毒性更高.
结论:
- 线粒体形态和膜潜力作为评估光毒性的可靠指标.
- 可以量化线粒体染料和照明条件的相对光毒性.
- 这些发现有助于优化超高分辨率成像协议,以尽量减少光毒性.
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