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人类牙纤维干细胞通过细胞间接触和膜信号传递来调节促炎性巨细胞

Monia Maccaferri¹, Alessandra Pisciotta¹, Gianluca Carnevale¹

¹Department of Surgery, Medicine Dentistry and Morphological Sciences with Interest in Transplant, Oncological and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.

Frontiers in immunology

|October 25, 2024

概括

人类牙纸干细胞 (hDPSCs) 通过减少TNFα和增加IL-10来调节促炎性巨细胞. 与hDPSCs直接的细胞接触会增强这些免疫调节效应,而不是对膜信号传递.

科学领域:

免疫学免疫学免疫学
干细胞生物学干细胞生物学
再生医学是一种再生医学.

背景情况:

巨细胞是诸如风湿性关节炎 (RA) 等炎症性疾病的关键参与者.
了解巨细胞的致病性对于开发新疗法至关重要.
人类牙纸干细胞 (hDPSCs) 具有免疫调节特性,并且很容易获得.

研究的目的:

评估hDPSCs调节巨细胞表型的能力.
为了比较直接的hDPSCs-巨接触与膜信号传递的效果.

主要方法:

单细胞分化为M1 (促炎) 和M2 (抗炎) 巨细胞.
巨细胞与hDPSCs直接或通过细胞培养插入在48小时内共同培养.
分析包括蛋白质和基因表达,细胞因子水平和免疫光.

主要成果:

hDPSCs通过直接接触和近信号传递减少了TNFα,并通过直接接触和近信号传递在促炎性巨细胞中增强了IL-10.
细胞对细胞接触显示出更明显的效应,降低NF-kB和COX-2表达和CD80/PD-L1同位化.
当hDPSC与巨细胞接触时,它们上调了NF-kB,COX-2,ICAM-1,PD-L1,FAS-L,TNFα和IFNγ.

结论:

hDPSC对促炎性巨细胞具有显著的免疫调节作用.
hDPSCs和巨细胞之间的直接细胞对细胞接触比对细胞信号更有效.
hDPSCs显示出作为炎症组织治疗剂的潜力.

关键词:

细胞培养细胞培养的方法细胞与细胞之间的接触是细胞间的接触.牙髓干细胞牙髓干细胞这是一种炎症炎症炎症炎症.巨细胞是什么?巨细胞是什么?偏神经信号传递系统

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