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在兽医临床细菌学中协调实验室方法:欧洲调查结果

Tom Koritnik¹, Iskra Cvetkovikj², Flavia Zendri^3,4

¹Department for Public Health Microbiology Ljubljana, Centre for Medical Microbiology, National Laboratory of Health, Environment and Food, Ljubljana, Slovenia.

Frontiers in microbiology

|October 25, 2024

概括

兽医微生物学实验室在细菌培养和抗菌素敏感性测试方面展示了多样化的实践. 协调方法和解释对于有效的抗微生物药物管理和耐药性监测至关重要.

科学领域:

兽医微生物学兽医微生物学
对抗生素耐药性的监测和监测
诊断实验室实践诊断实验室实践

背景情况:

兽医临床微生物学实验室对于抗菌药物管理,耐药性监测和感染预防至关重要.
在兽医细菌培养和报告方面缺乏协调的国际指导方针.

研究的目的:

调查欧洲兽医微生物学实验室中细菌培养和鉴定 (C&ID) 和抗菌感应性测试 (AST) 的现行实践和解释标准.
识别诊断工作流程中的变化和潜在改进领域.

主要方法:

欧洲兽医抗菌治疗优化网络 (ENOVAT) 分发了一项在线调查.
该调查重点关注C&ID,AST方法,以及动物细菌病原体的解释标准.
数据来自34个欧洲国家的241个实验室.

主要成果:

在C&ID和AST周转时间,方法 (生物化学试验,API,PCR,MALDI-TOFMS) 和解释标准 (EUCAST,CLSI,人类CBPs) 中观察到显著差异.
大多数实验室报告了定性AST结果 (S,R,I),并对特定的抗菌素耐药性机制 (如甲基西林耐药性和ESBL生产) 进行了近一半的查.
选择性报告AST结果是实践的,但类似比例的实验室没有指导抗生素选择的战略.

结论:

欧洲兽医微生物学实验室在C&ID和AST的方法和解释标准方面表现出相当大的异质性.
当前实践中的差距凸显了需要改善的专业培训,标准化的方法和协调的报告策略,用于AMR监测和管理.

关键词:

在 ENOVAT 中,您可以使用 ENOVAT.对抗微生物的敏感性测试.的细菌培养培养.细菌的鉴定细菌的鉴定统一化和化统一化和化方法论的方法论.兽医临床细菌学兽医临床细菌学

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