相关实验视频

Updated: Jun 9, 2025

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Published on: February 28, 2025

来自Codonopsis Pilosula的多糖化合物的结构特征以及与THP-1细胞上的免疫调节的结构-活性关系

Junjie Fu¹, Cong Chang¹, Jinyang Ren¹

¹School of Pharmacy, Hubei University of Chinese Medicine, No.16 Huangjiahu West Road, Hongshan District, Wuhan City, Hubei Province, China, 430065.

Chemistry & biodiversity

|October 25, 2024

概括

来自Codonopsis pilosula (CPs) 的多糖因其结构而表现出不同的免疫调节作用. 在CP中,较高的葡萄糖含量和延长链形状与增强的免疫增强特性相关.

科学领域:

* 生物化学 * 生物化学
* 免疫学免疫学
* * 自然产品化学自然产品化学

背景情况:

*来自Codonopsis pilosula (CPs) 的多糖因其免疫调节潜力而得到认可.
*CP的结构特征与免疫调节活性之间的具体关系仍未得到充分研究.

研究的目的:

* 调查不同CP分量的结构-活性关系.
* 阐明结构变异如何影响CP的免疫调节作用.

主要方法:

*使用染色学列分离方法分离了四种CP分量 (CPW,CPS0.2,CPS0.5,CPS1).
*分析了结构性质,包括单糖组成,链形状,分子量和聚合状态.
*通过在LPS诱导的THP-1细胞中测量细胞因子释放 (TNF-α,IL-1β) 来评估免疫调节作用.

主要成果:

* 在CP分量中观察到单糖组成,链形状,分子量和聚合状态的显著差异.
* 免疫调节效应在各分数之间有所不同,表明结构依赖的活性.
*较高的葡萄糖百分比,较大的颗粒大小和延长链形状与增强的免疫增强效应有关.

结论:

*CP的结构差异,特别是单糖组成,可以决定抗炎或促炎活动.
* 特定的结构特征,如高葡萄糖含量,大颗粒大小和延长链形状是强大的免疫增强的关键因素.
* 这项研究为确定Codonopsis pilosula polysaccharides的结构-活性关系提供了基础的理解.

关键词:

科多诺普西斯 (Codonopsis pilosula) 是一个的植物.形状的形成形状的形成这是一种免疫调节剂.聚糖是一种多糖.

更多相关视频

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate CDAP

Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate CDAP

Published on: June 14, 2021

相关概念视频

Biosynthesis of Polysaccharides

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...

Protein Glycosylation

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...

Formation of Lipopolysaccharides

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...