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相关概念视频

Proofreading01:31

Proofreading

6.2K
Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase...
6.2K
CRISPR01:59

CRISPR

49.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
49.7K
Mismatch Repair01:36

Mismatch Repair

39.9K
Overview
39.9K
Base Excision Repair01:54

Base Excision Repair

22.1K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
22.1K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.9K
Genome Copying Errors02:46

Genome Copying Errors

4.2K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
4.2K

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相关实验视频

Updated: Jun 9, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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[使用主要编辑纠正致病突变:概述]

Camille Bouchard1, Kelly Godbout1, Jacques P Tremblay1

  • 1Département de médecine moléculaire, Université Laval, Québec, Canada - Centre de recherche du CHU de Québec, Université Laval, Québec, Canada.

Medecine sciences : M/S
|October 25, 2024
PubMed
概括
此摘要是机器生成的。

主编辑是一种新的基因编辑技术,精确修改DNA. 本综述涵盖了其在疾病建模和基因治疗中的应用,强调了治疗用途的交付挑战.

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CIRCLE-Seq for Interrogation of Off-Target Gene Editing
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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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CIRCLE-Seq for Interrogation of Off-Target Gene Editing
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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物技术是生物技术.

背景情况:

  • 基因编辑技术正在迅速发展.
  • 总编辑代表了精确DNA修饰的重大创新.
  • 目前的基因编辑工具在准确性和范围方面面临限制.

研究的目的:

  • 审查Prime编辑技术的最新进展.
  • 探索Prime编辑在创建疾病模型中的应用.
  • 讨论Prime编辑在治疗遗传性疾病方面的潜力.
  • 在体内提供Prime编辑疗法的关键挑战.

主要方法:

  • 使用Cas9尼克酶与反转录酶融合.
  • 采用主要编辑指导RNA (pegRNA) 进行准和编辑.
  • 审查有关Prime编辑应用程序和交付策略的现有文献.

主要成果:

  • 主编辑能够精确地引入致病突变,用于疾病建模.
  • 它为纠正遗传疾病中引起疾病的突变提供了潜力.
  • 在实现有效和有针对性地将Prime编辑组件传递到特定器官的过程中,仍然存在重大挑战.

结论:

  • 总编辑是基础研究和治疗开发的强大工具.
  • 克服体内输送障碍对于实现Prime编辑的临床潜力至关重要.
  • 需要继续进行研究,以优化输送方法和扩大治疗应用.