通过金纳米粒子抗体结合物的色度测量免疫检测检测菌毒素
Vinayak Sharma1, Bilal Javed2, Hugh J Byrne2
1School of Food Science and Environmental Health, Technological University Dublin, D07 H6K8 Dublin, Ireland.
Biosensors
|October 25, 2024
概括
使用金纳米粒子 (AuNPs) 和抗体的新色度免疫传感器提供了一种快速,具有成本效益的方法,用于在低水平上检测亚毒素B1和泽拉伦. 这种生物传感探测器为食品安全应用提供敏感和特定的真菌毒素检测.
科学领域:
- 纳米技术 纳米技术
- 分析化学 分析化学
- 生物感应是一种生物感应.
背景情况:
- 黄金纳米粒子 (AuNPs) 由于其光学特性,稳定性和易于合,对生物感知有价值.
- 现有的毒素检测方法,如阿弗拉托克辛B1和泽拉伦,可能是复杂和昂贵的.
研究的目的:
- 开发一种简单,快速,经济高效的色度免疫传感探针,用于检测亚毒素B1和泽拉伦.
- 为了利用AuNPs的聚合诱导的光学变化用于真菌毒素量化.
主要方法:
- 抗 aflatoxin B1 和 zearalenone 的抗体被吸附到金纳米粒子 (AuNPs) 上.
- 该试验依赖于盐诱导的AuNPs聚合,该聚合由目标毒素的存在调节.
- 使用UV-可见-NIR光谱法来量化AuNP聚合导致的颜色变化.
主要成果:
- 开发的免疫检测仪检测到阿弗拉托克辛B1和泽拉伦,检测极限低至0.15 ng/mL.
- 颜色测定检测显示,在其他污染物存在的情况下,对菌毒素的高特异性.
- 该试验在使用人工增值样本进行测试时,证明了其在现实世界中的适用性.
结论:
- 这种基于局部表面等离子体共振 (LSPR) 的色度测量免疫探针为毒素检测提供了灵敏,快速和经济的替代方案.
- 易于制造和低检测极限使这种测试适合在食品安全和诊断领域广泛应用.
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