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碳和来源影响了NI-1的Trichoderma atroviride中的寄生反应

Víctor Javier García-Sánchez¹, Karina Lizbeth Sánchez-López¹, Juana Jazmín Esquivel Méndez¹

¹Unit for Basic and Applied Microbiology, Faculty of Natural Sciences, Autonomous University of Queretaro, Queretaro 76140, Mexico.

Journal of fungi (Basel, Switzerland)

|October 25, 2024

概括

特里科德玛亚特罗病毒的寄生性是由营养的可用性来调节的. 优选的碳和来源增强了寄生虫基因的表达,使宿主区分真菌和菌体.

科学领域:

* 菌类学和植物病理学
* 分子生物学和遗传学

背景情况:

* 寄生真菌,如Trichoderma物种,利用水解酶来降解宿主细胞壁.
* 众所周知,Trichoderma中的寄生体基因表达受到首选的碳和来源的抑制.
*了解营养调节对于利用Trichoderma的生物控制潜力至关重要.

研究的目的:

* 为了研究不同营养来源对Trichoderma atroviride分离NI-1的寄生活动的影响.
* 在不同营养条件下分析关键寄生性基因 (细胞氧水解酶,内葡萄糖酶,蛋白酶) 的表达模式.
* 确定T. atroviride能否区分真菌和菌体宿主,以及营养的可用性如何影响这种相互作用.

主要方法:

* Trichoderma atroviride分离NI-1在土豆乳糖 (PDA) 和使用各种碳和来源的最小介质上培养.
*对cbh1,cbh2,bgl3.1,pra1,prb1和ech42基因进行定量基因表达分析.
*与Phytophthora capsici,Botrytis cinerea和Rhizoctonia solani共同培养NI-1,以观察差异性基因诱导.

主要成果:

* 在与PDA上的Phytophthora capsici相互作用时,细胞基因水解酶 (cbh1,cbh2),内葡萄糖酶 (bgl3.1) 和蛋白酶 (pra1,prb1) 的基因表达较低.
* 作为碳源的德克斯特林和葡萄糖,以及作为源的氨,在最小的介质中显著提高了寄生虫基因的表达.
*特定的基因 (cbh1,cbh2) 被P. capsici独特诱导,表明从远处识别宿主. 其他基因 (pra1, ech42, prb1, bgl3.1) 在与不同病原体接触之前或接触时表现出不同的诱导模式.

结论:

* 营养素的可用性,特别是碳和来源,显著调节了Trichoderma atroviride的寄生反应.
* T. atroviride 显示出能够区分菌体 (P. capsici) 和真菌 (B. cinerea,R. solani) 主体的能力.
*这项研究突出了Trichoderma介导的寄生病中营养信号和宿主识别之间的复杂相互作用.

关键词:

生物控制生物控制催化剂抑制的催化剂抑制与真菌寄生虫症相关的基因主机传感感应的宿主传感器液溶性酶是液溶性酶的重要组成部分.寄生主义是一种寄生主义.

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