复原体对载荷蛋白CcSnc1的分类和运输调节了Corynespora cassiicola的生长,发育和致病性
Shuyuan Cheng1,2, Yunfei Long3, Xiaoyang Zhang4
1College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China.
Journal of fungi (Basel, Switzerland)
|October 25, 2024
概括
逆转激素复合体对于Corinespora cassiicola真菌中运输蛋白质至关重要. 这项研究表明,逆转激素.
科学领域:
- 菌类学 菌类学是指菌类学.
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 逆转基因复合物促进了在真核生物中内体到跨戈尔吉网络 (TGN) 的传输.
- 在有线状真菌中对逆转激素介导的货物蛋白质运输的研究,特别是关于生长,发育和致病性的研究,是有限的.
研究的目的:
- 为了研究复原体复合体在致病性真菌Corynespora cassiicola中的作用.
- 为了识别和描述参与真菌感染的逆转子载荷蛋白.
主要方法:
- 转录组分析,以评估在C. cassiicola入侵期间的复原体基因表达.
- 基因淘汰和补充,以确定逆转基因组件和货物的功能.
- 使用显微镜进行亚细胞局部化研究,以精确确定逆转激素复合体和载荷蛋白的位置.
- 同免疫沉以确认逆转激素子单元和货物蛋白之间的相互作用.
主要成果:
- 复原体复杂基因 (CcVPS35,CcVPS29,CcVPS26) 的表达在早期C. cassiicola入侵期间显著上调.
- 复原体复合物局部化到真空膜,内体和TGN.
- 复原体核心子单元CcVps35与载荷蛋白CcSnc1.1相互作用.
- 在CcVPS35被删除时,局部位于叶尖,内和戈尔吉的CcSnc1被错误地排序到真空降解途径.
- CcSnc1对于C. cassiicola的生长,发育和致病性至关重要.
结论:
- 逆转基因复合体介导了CcSnc1从内分体到C. cassiicola中的TGN的分类和运输.
- 这种逆转激素-CcSnc1通路对于真菌的生长,发育和病原性至关重要.
- 这些发现突显了真菌病原发生过程中的一个关键的囊泡运输机制.
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