展开蛋白质反应途径中的Aspergillus flavus hacA基因是宿主诱导的基因沉默的候选目标
1Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1100 Allen Toussaint Boulevard, New Orleans, LA 70124, USA.
Journal of fungi (Basel, Switzerland)
|October 25, 2024
概括
破坏真菌 hacA 基因对于展开的蛋白质反应至关重要,影响 Aspergillus flavus 的生存和发育. 哈卡的突变会影响形态和亚毒素的产生,这表明它是作物保护的目标.
科学领域:
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
- 遗传学 遗传学 是一个
背景情况:
- 展开的蛋白质反应 (UPR) 对细胞平衡,生长,发育和真菌中的毒性至关重要.
- 阿斯伯吉illus flavus hacA基因编码关键的转录因子域 (Bsc,bZip) 和一个独特的内子 (Nt20),调节UPR.
研究的目的:
- 通过使用CRISPR/Cas9.9来研究Aspergillus flavus hacA基因的基因型-表型关系.
- 阐明HacA基因内的Bsc编码,bZip编码和Nt20序列的功能意义.
主要方法:
- 使用CRISPR/Cas9基因编辑,在阿斯伯吉illus flavus hacA基因的BSC编码,bZip编码和Nt20序列中产生向突变.
- 分析了突变表型的生存率,殖民地形态,内质网膜 (ER) 应激敏感性和二次代谢物 (亚弗拉托克素,阿斯伯吉利酸,酸) 生产.
- 用RNA干扰 (RNAi) 来评估基因沉默对hacA转录水平和相关表型的影响.
主要成果:
- 在Bsc和bZip编码序列中的框架内突变是可行的,而框架外突变是致命的,表明本质性.
- 在Nt20内部突变导致低生存率 (约. 7%) 具有不同的殖民地形态 (硬质,混合,菌根).
- 硬质细胞和菌细胞突变体对ER压力的敏感性增加;菌细胞突变体失去了亚拉托克素生产能力,但保留了阿斯伯吉利酸和酸合成.
结论:
- 哈卡基因对于阿斯伯吉勒斯黄菌的生存,生长和发育至关重要,具有特定的域和内基因发挥关键作用.
- 改变hacA基因结构显著影响真菌形态,应激反应和像阿弗拉托克辛这样的真菌毒素的产生.
- 哈卡基因是开发宿主诱导的基因沉默策略的潜在目标,以控制真菌感染和减少作物污染.
关键词:
亚斯伯吉勒斯 (Aspergillus flavususus) 是一种有的植物.克里斯普尔是什么意思?克里斯普尔是什么意思?它们是RNAiRNAi.非洲毒素 (aflatoxin) 是一种毒素.bZip bZip 是一个简单的方法.主体诱导的基因沉默硬化组织 硬化组织二次新陈代谢二次新陈代谢不折叠的蛋白质反应反应更多相关视频
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