Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Proangiogenic effect and molecular mechanism of a new cytosporin derivative from the marine fungus Eutypella sp. D-1.

Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents·2026
Same author

The influence of extreme rainfall on crime: Spatiotemporal dynamics in Hunan Province, China.

PloS one·2026
Same author

Influence of Genetic Polymorphisms on Tacrolimus Pharmacokinetics in Whole Blood, Plasma, and Peripheral Blood Mononuclear Cells in Chinese Kidney Allograft Recipients.

Therapeutic drug monitoring·2026
Same author

Circulating tumor DNA as a prognostic biomarker in metastatic colorectal cancer: Post-hoc analysis of treatment response and survival outcomes in the ALTER-C-002 study.

Biomolecules & biomedicine·2026
Same author

Aptamer-Based Dual-Cascade Signal Amplification System Lights up G-Quadruplex Dimers for Ultrasensitive Detection of Domoic Acid.

Marine drugs·2026
Same author

Pharmacokinetics of mycophenolic acid in plasma and peripheral blood mononuclear cells and its relationship with activity of inosine monophosphate dehydrogenase in Chinese adult kidney allograft recipients.

Frontiers in pharmacology·2025

相关实验视频

Updated: Jul 13, 2026

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

9.3K

布雷维毒素阿普塔默的选择和生物层干扰测量生物传感器应用.

Bo Hu1, Sheng-Qun Ouyang2, Yu-Ping Zhu2

  • 1Naval Medical Center of PLA, Naval Medical University, Shanghai 200433, China.

Toxins
|October 25, 2024
PubMed
概括

研究人员开发了一种新型的食欲传感器,用于检测Brevetoxin-1 (PbTx-1),一种强大的海洋神经毒素. 这种生物传感器为监测神经性贝类中毒 (NSP) 的传统方法提供了一种特定,可靠和敏感的替代方案.

关键词:
一个aptamer的应用程序.一个适应传感器.生物层干涉度测试是生物层干涉度测试.简短的毒素短信

更多相关视频

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription
07:18

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription

Published on: July 26, 2019

13.6K
Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
08:07

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry

Published on: January 17, 2025

1.3K

相关实验视频

Last Updated: Jul 13, 2026

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

9.3K
Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription
07:18

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription

Published on: July 26, 2019

13.6K
Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
08:07

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry

Published on: January 17, 2025

1.3K

科学领域:

  • 检测海洋毒素的检测方法
  • 生物传感器技术技术
  • 分子识别分子识别

背景情况:

  • 毒素 (PbTxs) 是一种强大的海洋神经毒素,会引起神经学性贝类中毒 (NSP).
  • 增加PbTxs的地理分布需要改进的监测方法.
  • 当前的分析方法面临着伦理和技术方面的挑战.

研究的目的:

  • 开发一种新型的食欲传感器,用于特定检测布雷维托克辛-1 (PbTx-1).
  • 解决现有的海洋毒素检测技术的局限性.
  • 为监管监督提供可靠的替代方案.

主要方法:

  • 同时选择Brevetoxin-1 (PbTx-1) 和Brevetoxin-2 (PbTx-2) 的阿帕特马.
  • 使用高 afinity PbTx-1 吸收体 (A5-S3G) 构建一个生物层干扰测量 (BLI) 生物传感器.
  • 通过切断和突变优化阿巴酶序列,以提高结合亲和力.

主要成果:

  • 成功选择了对PbTx-1具有高亲和力和特异性的DNA体.
  • 开发了一种无标签的BLI口味传感器,其检测范围为100-4000nm,LOD为4.5nm,用于PbTx-1.
  • 具有很高的特异性,没有与PbTx-2或其他海洋毒素的交叉反应,在贝类样本中具有很好的稳定性.

结论:

  • 开发的 BLI 适应传感器是一个有希望的,创新的工具,用于特定和可靠的 PbTx-1 检测.
  • 这种口味传感器为海洋毒素监测的传统免疫学方法提供了可行的替代方案.
  • 这些发现支持适应传感器在贝类安全监管监测制度中的应用.