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在急性损伤中用于无标签检测miRNA的分割G-四重复编程可回收AIE生物传感器

Wenjie Ma^1,2, Tingfei Xie¹, Jinyan Li²

¹Department of Nephrology, The Second Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518000, China.

Analytical chemistry

|October 25, 2024

概括

一种新型的AIE生物传感器使用分裂的G-四重复合体,可用于敏损伤 (AKI) 诊断微RNA (miRNA) 的敏感,无标签的检测. 这种基于G-quadruplex的生物传感器为早期AKI查提供了一种简单有效的方法.

科学领域:

生物医学工程生物医学工程
分子诊断学分子诊断学
纳米技术纳米技术

背景情况:

诊断急性损伤 (AKI) 需要敏感和特定的生物标志物.
微RNAs (miRNAs) 是AKI的新兴生物标志物,需要强大的检测方法.
目前的检测方法往往缺乏灵敏度,特异性,或是取决于标签.

研究的目的:

合理设计一个可回收的AIE生物传感器,用于无标签的miRNA检测.
开发一种针对急性损伤 (AKI) 的敏感和特定诊断工具.
为了增强信号放大,利用分裂的G-四重复结构.

主要方法:

设计一个分割的G-四重复探头,以防止光火.
利用目标诱导的G-四重复形成来激活分子信标.
使用λ-外核酶 (λ-Exo) 进行目标回收和信号放大.

主要成果:

在目标miRNA结合时,AIE生物传感器显示了一个无标签的"ON"光信号.
实现了对miRNA-21的高度敏感的定量检测,降至10.36 fM.
显示出出色的特异性,区分单个不匹配的目标.

结论:

开发的基于G-quadruplex的AIE生物传感器有效用于敏感和特定的miRNA检测.
生物传感器显示出对非侵入性,简单和高效的AKI查和早期诊断的承诺.
目标回收机制显著提高了检测灵敏度,提供了临床潜力.

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