人类胚胎膜支架增强脂肪介质细胞 stromal 细胞线粒体生物能量促进他们的再生能力
Ahmed M Abou-Shanab1, Ola A Gaser1, Mariam Waleed Soliman1
1Center of Excellence for Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, Giza, 12578, Egypt.
Molecular and cellular biochemistry
|October 25, 2024
概括
人类胚胎膜支架显著增强介质细胞 stromal 细胞 (MSC) 活力,增殖和分化潜力. 这些支架优化MSC代谢,促进组织工程应用的再生能力.
科学领域:
- 生物材料科学 生物材料科学
- 干细胞生物学 干细胞生物学
- 组织工程是组织工程.
背景情况:
- 人类胚胎膜 (hAM) 在组织工程中被用作支架,以支持干细胞.
- 介酶体 stromal 细胞 (MSCs) 具有对于治疗应用至关重要的再生潜力.
研究的目的:
- 在3D环境中研究培养MSCs在hAM支架上的分子和生化影响.
- 评估hAM如何影响MSC功能,新陈代谢和分化能力.
主要方法:
- 在脱细胞化hAM支架上培养脂肪衍生的MSCs (AMSCs) 和骨髓衍生的MSCs (BMSCs).
- 分析细胞活力,增殖,细胞灭亡抵抗,MSC标记物表达和分化潜力.
- 评估细胞呼吸,糖解,线粒体氧化酸化 (OXPHOS),活性氧物种 (ROS) 生产和尿素循环活性.
主要成果:
- hAM支架显著改善了MSC的生存能力,增殖,亡抵抗力和MSC标记物的表达.
- 培养的MSCs表现出改变的亲血管生成和炎症标志物表达,增强了脂肪和骨质分化.
- hAM通过调节糖解,改变OXPHOS,ROS产量,抗氧化能力和促进尿素循环来调节MSC新陈代谢.
结论:
- hAM 作为一个仿生支架,有利地增强体外MSC功能.
- 观察到的代谢和功能改善支持hAM培养的MSC在组织再生中的潜在临床应用.
相关概念视频
01:09Introduction to Fibroblasts
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
01:10Zygotic Development And Stem Cell Formation
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...