用HemoCue测定血红素度的毛细血管滴,毛细血管聚合和静脉血液样本:一种测量系统分析
Ignacio Méndez-Gómez-Humarán1, Vanessa De la Cruz-Góngora2,3, Omar Dary4
1Center for Research in Mathematics CIMAT, Aguascalientes Unit, Aguascalientes, Mexico.
PloS one
|October 25, 2024
概括
个体的变化,而不是HemoCue装置,显著影响血红蛋白度[Hb]的测量. 在受控设置中,HemoCue系统显示出高可靠性,总测量误差为4.2%.
科学领域:
- 临床化学 临床化学
- 生物医学工程 生物医学工程
- 实验室医学 实验室医学
背景情况:
- 血红蛋白度[Hb]测量对于诊断贫血和监测健康至关重要.
- 影响HemoCue设备性能和Hb测量变异性的因素尚未得到充分记录.
- 了解变异源对于准确的临床解释至关重要.
研究的目的:
- 使用HemoCue量化不同错误来源对血红蛋白度[Hb]测量的贡献.
- 在各种样本类型中评估HemoCue Hb测量的准确性和精度.
- 在受控的实验环境中确定HemoCue系统的整体可靠性.
主要方法:
- 采用了一种重复测量设计,共9名参与者,3名HemoCue设备和3名现场工作人员.
- 进行一致性分析以评估Hb测量的准确性和精度.
- 利用线性混合模型估计偏差并量化设备,人员和个体变化的贡献.
主要成果:
- 个体变异占Hb测量的总变异的95.8%.
- HemoCue 设备对测量误差贡献了 2.1%,而现场工作人员贡献了 0.4%.
- 在使用静脉EDTA样本进行校正后,毛细管滴的平均差异为0.585g/dL,聚合毛细管样本没有显著差异.
结论:
- 在受控环境中,HemoCue Hb测量系统可靠,总测量误差为4.2%.
- 个体生物变异是HemoCue Hb测量变异的主要来源.
- 在受控环境中正确使用时,HemoCue设备显示了可接受的精度和准确性.
更多相关视频
08:44An In Vitro Hemodynamic Loop Model to Investigate the Hemocytocompatibility and Host Cell Activation of Vascular Medical Devices
Published on: August 21, 2020
7.0K
07:24A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy
1.7K
相关概念视频
01:24Hemoglobin
3.4K
Hemoglobin is a globular protein made up of four subunits. Two of these subunits are alpha chains, and the other two are beta chains. Each subunit contains a molecule of heme, which has an iron atom and can bind to oxygen. When an oxygen molecule binds to one heme group, it changes the shape of hemoglobin, making it easier for the other heme groups to bind oxygen as well.
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
3.4K
01:27Oxygen Transport in the Blood
2.5K
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
2.5K
01:20Capillary Electrophoresis: Instrumentation
190
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
190
01:20Electrophoresis: Overview
1.5K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
There...
1.5K