相关实验视频
Updated: Jun 9, 2025
13:47
Chromatin Immunoprecipitation ChIP using Drosophila tissue
Published on: March 23, 2012
25.1K
基因组甲基转移酶NSD调节了Drosophila染色体4上的基因沉默机制
Donghee Ko1, Kyungju Nam1, Byungjun Kang1
1Department of Biological Sciences, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.
Biochemical and biophysical research communications
|October 25, 2024
概括
核受体结合SET域蛋白 (NSD) 功能在Drosophila中的丧失破坏了4号染色体的异质染色体. 这种表观遗传调节涉及减少HP1a结合,影响基因沉默.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 染色体生物学 染色体生物学
- 发展生物学 发展生物学
背景情况:
- 核受体结合性SET域蛋白 (NSD) 是调节H3K36甲基化作用的基因组甲基转移酶.
- 基因突变对维护染色质结构和基因表达至关重要.
- 德罗斯菲拉4号染色体在很大程度上是异色染色体,并受到特定的调节机制的影响.
研究的目的:
- 为了研究NSD蛋白在调节Drosophila的转录和异色素蛋白形成中的作用.
- 阐明NSD功能丧失对4号染色体表观遗传景观的影响.
主要方法:
- 转录组分析 (RNA-seq) 来评估在NSD删除后的基因表达变化.
- 使用小白色 (mw+) 转基因评估染色质可访问性的位置效应变异试验.
- 染色体免疫沉 (ChIP) 检测异染色体标志物结合 (HP1a,H3K9me2).
主要成果:
- 脱离NSD导致4号染色体上基因活化增加,特别是在异色染色区域.
- 丧失NSD功能促进了4号染色体上微型白色转基因表达,表明沉默减少.
- NSD删除减少了异染色素标记物HP1a和H3K9me2与染色体4的结合.
结论:
- NSD蛋白对于保持Drosophila染色体4上的异性染色素结构至关重要.
- 失去NSD功能会通过减少HP1a结合来破坏异色素蛋白,从而导致基因表达的改变.
- NSD充当了一种表观遗传调节剂,参与4号染色体的沉默.
关键词:
染色体4的第4个染色体这种植物是Drosophila.表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.H3K3636 在线阅读异性染色是一种异性染色.这是NSD NSD.位置效应变化的位置效应.更多相关视频
10:09Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
Published on: January 26, 2018
7.4K
10:13Immunofluorescent Staining for Visualization of Heterochromatin Associated Proteins in Drosophila Salivary Glands
Published on: August 21, 2021
3.7K
相关概念视频
02:32Position-effect Variegation
6.3K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.3K
02:32Histone Modification
13.1K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.1K
02:25Spreading of Chromatin Modifications
8.2K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.2K
01:32Chromatin Modification in iPS Cells
1.6K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.6K
02:54Nucleosome Remodeling
9.0K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
9.0K
02:50Dosage Compensation
6.1K
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
6.1K