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不经预处理的果皮的高固体糖化通过量身定制的细胞酶
Weiqing Yang1, Jing Li2, Zhiliang Yao2
1School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, China.
International journal of biological macromolecules
|October 25, 2024
概括
果皮是生物炼油厂的一个有前途的纤维素生物质. 这项研究优化了酶尾酒和食批量策略,以有效地从类废物中生产高固体糖.
科学领域:
- 生物质转换生物质转换
- 生物炼油技术 生物炼油技术
- 可再生能源可再生能源是可再生能源.
背景情况:
- 果皮是丰富的纤维素生物质,含有低水平的纤维素和高糖分.
- 它们代表了一个有价值的,未被充分利用的资源,用于生物炼油应用.
- 没有预处理的酶可访问性是一个关键优势.
研究的目的:
- 开发一种优化的酶过程,用于果皮的高固体糖化.
- 使用响应表面方法 (RSM) 制定一个量身定制的细胞质尾酒.
- 研究一种料批量策略,以实现高基板负荷和糖产量.
主要方法:
- 响应表面方法 (RSM) 用于优化细胞质尾酒的组成.
- 开发一种定制的酶混合物,包括β-葡萄糖酶,半细胞酶,脂和Tween 80.
- 实施料批量策略,以增强糖化过程中的基质负荷.
主要成果:
- 一个优化的细胞酶尾酒被配制成特定的酶和表面活性剂度.
- 通过类废物达到22%的高固体负载.
- 获得总糖度为123.84g/L,从生物质中获得93.12%的糖.
结论:
- 类废物在没有预处理的情况下可以通过酶来获取,验证了其作为原料的潜力.
- 优化的工艺证明了在生物炼油厂中有效利用果皮用于糖生产.
- 这项研究为从类废物中开发可持续的糖平台提供了基础.
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