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相关概念视频

Mitochondrial Precursor Proteins01:39

Mitochondrial Precursor Proteins

2.5K
Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70  chaperones are targetted to TOM20-TOM22 receptor complexes.
Most of the mitochondrial...
2.5K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

3.0K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.0K
Mitochondrial Protein Sorting01:39

Mitochondrial Protein Sorting

4.3K
Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death.  Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
4.3K
Protein Transport into the Inner Mitochondrial Membrane01:34

Protein Transport into the Inner Mitochondrial Membrane

3.6K
Nuclear encoded mitochondrial precursors are imported to the inner membrane in a multistep process involving two separate translocons, TIM22 and TIM23. TIM23 is a cation-selective pore that remains closed by the N terminal segment of the protein. Negative charges on the TIM23 act as a receptor for the incoming precursor, pulling the positively charged matrix-targeting sequence for peptide insertion and translocation.
Transport of mitochondrial precursors across the TIM23 channel is driven by...
3.6K
Energy to Drive Translocation01:37

Energy to Drive Translocation

2.0K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
2.0K
Porin Insertion in the Outer Mitochondrial Membrane01:12

Porin Insertion in the Outer Mitochondrial Membrane

2.8K
Porins are beta-barrel proteins translocated to the mitochondrial outer membrane through the TOM complex into the intermembrane space. Porin precursors bind TIM chaperones within the intermembrane space and are guided to the Sorting and Assembly Machinery complex or SAM complex on the outer mitochondrial membrane.
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
2.8K

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相关实验视频

Updated: Jun 9, 2025

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria
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Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria

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通过mePRODmt蛋白质学量化线粒体蛋白质进口.

Süleyman Bozkurt1, Bhavesh S Parmar1, Christian Münch2

  • 1Institute of Molecular Systems Medicine, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany.

Methods in enzymology
|October 25, 2024
PubMed
概括

我们开发了线粒体特定的多重增强蛋白动力学 (mePRODmt) 来研究蛋白质如何进入线粒体. 这种新方法提供了线粒体蛋白质稳定性的动态视图,改善了疾病研究.

关键词:
质谱测量质量谱测量线粒体中的线粒体.线粒体蛋白质的进口蛋白质组学是指蛋白质组学.这就是SILACLAC.在TMT的多媒体平台上.翻译 翻译 翻译 翻译这就是mePROD.在这里,pSILAC.

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Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification
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Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae
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相关实验视频

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Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria
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Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria

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Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification
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Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae
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科学领域:

  • 细胞生物学 细胞生物学
  • 生物化学 生物化学
  • 蛋白质组学是指蛋白质组学.

背景情况:

  • 线粒体蛋白质的进口对于细胞功能和恒常状态至关重要.
  • 线粒体蛋白质进口的失调与各种疾病有关.
  • 现有的方法缺乏动态分辨率,无法完全捕获线粒体蛋白质稳定.

研究的目的:

  • 引入线粒体特异多重增强蛋白动力学 (mePRODmt) 用于研究短暂的线粒体蛋白质进口.
  • 为mePRODmt技术提供详细的协议.
  • 为了增强对线粒体蛋白质贩运和蛋白质静止的理解.

主要方法:

  • 在细胞培养中利用脉冲稳定同位素标记与氨基酸 (pSILAC) 蛋白质组学.
  • 包含重型SILAC标记作为增强线粒体信号的增强剂.
  • 在多重蛋白质组学中使用双重质量标签 (TMT) 进行等离体标签.
  • 详细的液体染色学-质谱学 (LC-MS) 设置用于记者离子量化.
  • 为pSILAC-TMT数据建立了一个数据分析管道.

主要成果:

  • 该mePRODmt协议使线粒体蛋白质进口的动态分析成为可能.
  • 该方法允许线粒体蛋白质的多重定量蛋白质组.
  • 优化的LC-MS和数据分析策略确保了准确的量化.

结论:

  • mePRODmt提供了一种强大的,动态的方法来研究线粒体蛋白质进口.
  • 这种技术推动了线粒体蛋白质稳定及其在疾病中的作用的研究.
  • 该议定书为该领域的研究人员提供了一个全面的框架.