相关实验视频

Updated: Jun 9, 2025

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Published on: February 28, 2025

通过激活自细胞,IL-27减弱了由Mycobacterium tuberculosis引起的巨细胞损伤和炎症

Yushan Zhou¹, Yuxuan Zhang¹, Yanli Li¹

¹Respiratory and Critical Care Medicine Ward 1, Yan'an Hospital Affiliated to Kunming Medical University, Kunming, 650051, Yunnan, China.

In vitro cellular & developmental biology. Animal

|October 25, 2024

概括

介素-27 (IL-27) 减少了感染Mycobacterium tuberculosis (Mtb) 的巨细胞的炎症和细胞损伤. 这种细胞因子激活了自,为肺结核 (PTB) 提供了潜在的新治疗点.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
传染性疾病传染性疾病

背景情况:

肺结核 (PTB) 患者表现出高的IN-27 (IL-27) 水平.
在受感染的巨细胞中,IL-27信号通路显示出抗Mycobacterium结核病 (Mtb) 的特性.
对于IL-27对Mtb的保护作用的精确分子机制尚不清楚.

研究的目的:

阐明IL-27增强巨细胞对Mtb感染的抵抗力的分子机制.
调查IL-27在调节巨细胞亡和炎症中的作用.

主要方法:

产生了单细胞衍生的巨细胞 (MDM) 和THP-1衍生的巨细胞样细胞 (TDM).
巨细胞被Mtb菌株H37Rv感染,并预先接受IL-27.
通过流式细胞计,ELISA和西式涂抹评估了巨损伤,亡和炎症标志物;IL-27受体 (IL-27Ra) 被沉默以确认机制.

主要成果:

在巨细胞中,IL-27预条件显著降低了Mtb诱导的亡和炎症性细胞因子产生 (TNF-α,IL-1β,IL-6).
IL-27促进了抗亡蛋白 (Bcl-2) 和抗炎细胞因子 (IL-10,IL-4) 的表达.
通过抑制TLR4/NF-κB信号和激活PI3K/AKT,IL-27激活了自,从而抑制了Mtb诱导的巨细胞亡和炎症.

结论:

通过自激活,IL-27可缓解Mtb诱导的巨细胞损伤和炎症.
IL-27显示出作为肺结核治疗的新型治疗点的潜力.

关键词:

结核病菌菌菌的结核病菌的结核病菌.在IL-27和IL-27之间.巨细胞是一个巨细胞.在PI3K/AKT中使用.在 TLR4/NF-κBB 中.结核病是一种疾病.

更多相关视频

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection

Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection

Published on: September 18, 2020

Author Spotlight: Optimizing CFU Determination for Efficient Assessment of TB Vaccine Efficacy and Antigen Presentation Analysis

Author Spotlight: Optimizing CFU Determination for Efficient Assessment of TB Vaccine Efficacy and Antigen Presentation Analysis

Published on: July 28, 2023

相关概念视频

Pulmonary Tuberculosis II

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...

Defense Against Bacterial Pathogens

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...