通过iPSC衍生的心房肌细胞建立的心房动模型的最佳参考基因的识别
Lei Li1,2, Zijuan Zhao3, Zihao Liu1,2
1Department of Cardiovascular Surgery, The Affiliated Hospital of Southwest Medical University, NO25, Taiping Street, Luzhou City, 646000, China.
BMC genomics
|October 25, 2024
概括
研究人员使用人类诱导的多能干细胞干细胞衍生的心房肌细胞 (hiPSC-AMs) 开发了一种新的体外心房动 (AF) 模型. 这种模型通过电生理学和成像验证,确定ABL1为AF研究的优越参考基因.
科学领域:
- 心脏病学 心脏病学
- 干细胞生物学 干细胞生物学
- 电力生理学 电力生理学
背景情况:
- 心房动 (AF) 是一种常见的心律失常,具有复杂的机制.
- 需要可靠的体外模型来研究AF病理生理学.
- 目前的 in vitro AF 模型缺乏一致性,阻碍了研究.
研究的目的:
- 建立一个可复制的心房动 (AF) 试验室模型.
- 为了验证人类诱导的多能干细胞衍生的心房肌细胞 (hiPSC-AMs) 用于AF建模.
- 在AF模型中确定最佳基因基因表达分析的参考基因.
主要方法:
- 使用GiWi方法生成hiPSC-AMs,通过补丁,免疫光和流细胞计验证.
- 通过热应力 (HS) 在hiPSC-AM中建立了一个AF模型.
- 使用补丁和成像评估AF模型特征;评估了29个参考基因的稳定性.
主要成果:
- hiPSC-AMs表现出心房肌细胞动作潜力,并表达出心房特异性标记物.
- 热应激诱导了类似于AF的变化:打击频率增加,动作潜能持续时间缩短,过渡频率升高.
- ABL1,RPL37A,POP4,RPL30和EIF2B1是最稳定的参考基因;ABL1显示出比GAPDH更高的正常化效率.
结论:
- 热应力诱导的hiPSC-AMs模型是AF研究的一个可行的工具.
- 与GAPDH相比,ABL1是这个hiPSC-AMs AF模型中正常化的更有效,更可靠的参考基因.
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