VvD14c-VvMAX2-VvLOB/VvLBD19模块参与了葡萄树根架构的以strigolactone为媒介的调节
Yan Xu1, Zhengxin Lv1, Muhammad Aamir Manzoor1
1Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Dongchuan Road No. 800, Shanghai, 200240, P. R. China.
Molecular horticulture
|October 26, 2024
概括
斯特里戈拉克顿 (SLs) 调节葡萄树根结构. VvD14c蛋白与VvMAX2相互作用,通过VvD14c-VvMAX2-VvLOB/VvLBD19通路影响横向根的发展.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 斯特里戈拉克顿 (SLs) 是植物激素,对各种发育过程至关重要.
- D14蛋白作为SL信号通路中的关键受体.
- 在葡萄树根架构中VvD14的特定作用仍然在很大程度上未被描述.
研究的目的:
- 研究VvD14在葡萄树根发育中的作用.
- 阐明SL介导的葡萄树根架构调节的分子机制.
- 为了确定SL信号通路中的关键蛋白相互作用,在Vitis vinifera.
主要方法:
- 在不同的葡萄树组织和发育阶段对VvD14异型的基因表达分析.
- 应用GR24 (SL类比) 来评估其对根形态和VvD14c表达的影响.
- 生物化学试验 (例如,蛋白质结合,酵母双杂交) 来确定蛋白质-蛋白质相互作用.
- 在葡萄藤和阿拉比多普西斯的基因操纵 (过度表达和突变分析) 中.
主要成果:
- 与其他异构体相比,VvD14c在葡萄树组织和果实阶段表达更高.
- GR24的应用促进了偶然的根生长,但抑制了横向根 (LR) 延长和密度,同时上调VvD14c.
- GR24对VvD14c表现出强烈的结合亲和力,而VvD14c与VvMAX2以GR24依赖的方式相互作用.
- 在Arabidopsis和葡萄藤中,VvD14c和VvMAX2调节的LR数量,密度和初级根延伸的过度表达.
- 发现VvMAX2与VvLOB和VvLBD19相互作用,这表明它在积极调节LR密度方面发挥了作用.
结论:
- 斯特里戈拉克顿在调节葡萄树根结构方面发挥着重要作用.
- VvD14c-VvMAX2-VvLOB/VvLBD19模块可能参与中介SL对根部发育的影响.
- 这项研究为葡萄树根生长调节的分子基础提供了新的见解.
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