基托桑生物矿物化与离子合的纳米-氧酸盐调节骨质细胞代谢和DNA损伤
Franco Furlani1,2,3, Matilde Clarissa Malfatti4,5, Alfredo Rondinella6
1Department of Medicine, University of Udine, Piazzale Kolbe 4, Udine, 33100, Italy. franco.furlani@uniud.it.
Journal of biological engineering
|October 26, 2024
概括
用或铁离子杂的生物矿物化酸 (HA) 纳米粒子在酸盐模板上合成. 这些纳米粒子增强了细胞代谢,但诱导了DNA损伤,特别是与素结合时,这表明在癌症治疗中具有潜在的应用.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术 纳米技术
- 细胞生物学 细胞生物学
背景情况:
- 酸 (HA) 是骨再生的关键生物材料.
- 生物矿物化提供了一种在聚合物模板上合成HA纳米粒子的方法.
- 离子兴奋剂和生物矿物化对细胞反应的影响仍未得到充分研究.
研究的目的:
- 在酸盐模板上合成和表征 (MgHA) 和铁 (FeHA) 离子合的HA纳米粒子.
- 研究这些纳米颗粒对人类骨质母细胞类细胞 (MG63和HOBIT) 的影响.
- 评估对细胞代谢,增殖,氧化还原状态和DNA损伤的影响.
主要方法:
- 生物矿物化酸盐-HA纳米粒子 (MgHA,FeHA) 的合成.
- 纳米粒子的物理化学和形态特征.
- 细胞反应的体外评估:新陈代谢,增殖,ROS生产,Peroxiredoxin氧化和DNA损伤 (COMET试验, γH2AX焦点).
主要成果:
- 在奇托上成功合成了低晶度,离子合的HA纳米颗粒.
- 纳米粒子增强了细胞代谢,但稍微减少了细胞的增殖.
- 与素合铁合的HA显示了反应性氧物种 (ROS) 的轻微增加.
- 所有配方都诱导了DNA损伤,由酸盐放大,表明细胞吸收增强.
结论:
- 生物矿物化离子合HA纳米颗粒调节细胞代谢和增殖.
- 奇托增强HA纳米颗粒的内化和生物活性,包括诱导DNA损伤.
- 这些素-HA复合物的DNA损伤潜力值得考虑生物材料设计,包括潜在的抗癌应用.
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