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Updated: Jun 9, 2025

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
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Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

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一个细菌平台,用于研究 SCF-Type E3 Ubiquitin Ligases 定下的 Ubiquitin 级联

Zuo-Xian Pu1,2, Jun-Li Wang1,2, Yu-Yang Li1,2

  • 1Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.

Biomolecules
|October 26, 2024
PubMed
概括

一个新的细菌共表达系统使得SKP1-CUL1-F-Box (SCF) 类型E3无素连接酶的研究成为可能. 这种方法通过表达大肠杆菌中所有必要的成分来简化检测无处不在活性,从而促进对这些关键酶的研究.

关键词:
在AUX/IAAs方面,双人表达系统是双人表达系统在TIR1的自动无处不在.同表达的重组蛋白质.检测到无处不在的化.

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