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相关概念视频

Histone Modification02:32

Histone Modification

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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
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Expression Analysis of Mammalian Linker-histone Subtypes
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使用向蛋白质组学量化素H1亚型的量化.

Jordi López-Gómez1, Laura Villarreal2,3, Marta Andrés1

  • 1Biochemistry and Molecular Biology Department, Biosciences Faculty, Autonomous University of Barcelona, 08193 Bellaterra, Spain.

Biomolecules
|October 26, 2024
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概括

基斯H1亚型调节色素. 一种新的质谱分析量化H1水平,揭示慢性髓性白血病 (CML) 治疗反应的潜在生物标志物.

关键词:
癌症生物标志物 癌症生物标志物慢性骨髓性白血病 慢性骨髓性白血病功能差异化的功能差异化.基因组 H1 基因组 H1产生对伊马替尼布的耐药性.平行反应监测并行反应监测.

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Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue
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科学领域:

  • 分子生物学分子生物学
  • 蛋白质组学是指蛋白质组学.
  • 癌症研究 癌症研究

背景情况:

  • 希斯H1亚型调节染色质结构,它们在癌症中的变化水平表明它们可能是生物标志物.
  • 在H1补充的变化 (体质H1s的比例) 暗示功能特异性.

研究的目的:

  • 开发和验证基于质谱的并行反应监测 (PRM) 试验,用于量化人类Histone H1亚型.
  • 研究H1补充变化的潜力,作为慢性髓性白血病 (CML) 的生物标志物.

主要方法:

  • 开发基于质谱的高度特异的并行反应监测 (MS-PRM) 试验,使用每个H1亚型的独特.
  • 在人类细胞系 (HeLa,K562,T47D) 上验证MS-PRM试验的可重复性,灵敏性和精度,与电泳和西斑数据相比.
  • 应用验证的MS-PRM测定方法来量化H1补充剂在健康个体和CML患者的外周血液样本中的应用.

主要成果:

  • 该MS-PRM测定显示出高的可重现性,灵敏度和精度在量化H1亚型跨不同的人类细胞系.
  • 在慢性髓性白血病 (CML) 患者中,初步数据表明意马提尼布响应者和不响应者之间的H1补充物存在差异.
  • 来自MS-PRM试验的量化值与电泳术和西式涂抹等既定方法一致.

结论:

  • 开发的MS-PRM测定是一种可靠的工具,用于精确量化Histon H1亚型.
  • 在CML患者中观察到的H1补充的差异表明,H1亚型可以作为预测性生物标志物来预测意马替尼的反应.
  • 需要进一步的研究来探索H1补充成分的临床实用性,以预测CML的治疗结果.