Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

7.3K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
7.3K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

4.7K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.7K
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

5.7K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.7K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.7K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

8.7K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.7K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

4.9K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
4.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

MAVS as a Key Regulator of Tumor Proliferation, Survival, the Tumor Microenvironment, and Immunity.

Biomolecules·2026
Same author

Disrupting VDAC1-tubulin interaction uncovers crosstalk between mitochondrial and microtubule functions with implication to cancer therapy.

Cellular and molecular life sciences : CMLS·2026
Same author

p53 Interacts with VDAC1, Modulating Its Expression Level and Oligomeric State to Activate Apoptosis.

Biomolecules·2026
Same author

Resveratrol's Pro-Apoptotic Effects in Cancer Are Mediated Through the Interaction and Oligomerization of the Mitochondrial VDAC1.

International journal of molecular sciences·2025
Same author

Correction to: Elevated serum mtDNA in COVID‑19 patients is linked to SARS‑CoV‑2 envelope protein targeting mitochondrial VDAC1, inducing apoptosis and mtDNA release.

Apoptosis : an international journal on programmed cell death·2025
Same author

Annexin A5 controls VDAC1-dependent mitochondrial Ca<sup>2+</sup> homeostasis and determines cellular susceptibility to apoptosis.

The EMBO journal·2025

相关实验视频

Updated: Jun 9, 2025

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

20.5K

通过使线粒体守门员VDAC1沉默来解码癌症.

Tasleem Arif1,2, Anna Shteinfer-Kuzmine3, Varda Shoshan-Barmatz3,4

  • 1Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.

Biomolecules
|October 26, 2024
PubMed
概括

沉默电压依赖性离子通道1 (VDAC1) 逆转了癌症.

关键词:
在 VDAC1 中,VDAC1 是代谢 代谢 代谢 代谢线粒体中的线粒体.siRNA 是一个RNA.干细胞是干细胞的组成部分.

更多相关视频

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
09:24

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

Published on: August 12, 2015

9.0K
Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples
09:57

Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples

Published on: May 10, 2017

7.6K

相关实验视频

Last Updated: Jun 9, 2025

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

20.5K
Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
09:24

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

Published on: August 12, 2015

9.0K
Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples
09:57

Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples

Published on: May 10, 2017

7.6K

科学领域:

  • 线粒体生物学 线粒体生物学
  • 癌细胞的新陈代谢
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 线粒体调节关键的细胞过程,包括新陈代谢和亡.
  • 电压依赖性离子通道1 (VDAC1) 是线粒体的守门员,控制离子和代谢物运输.
  • 在许多癌症中,VDAC1过度表达,并与华堡效应有关.

研究的目的:

  • 研究向癌症中的VDAC1的治疗潜力.
  • 探索VDAC1沉默对癌细胞表型和瘤微环境的影响.

主要方法:

  • 在各种瘤类型中使用小干扰RNA (siRNA) 抑制VDAC1表达.
  • 对代谢重新连接,致癌性质,瘤生长,侵袭性,干性和上皮-介质酶过渡的分析.
  • 评估瘤微环境,血管新生和细胞外基因表达的变化.
  • 对表观遗传酶和基因组修饰的VDAC1枯竭效应的评估.

主要成果:

  • 沉默VDAC1逆转了癌细胞的代谢重编程和瘤性质.
  • 观察到瘤生长,侵袭性和茎的减少.
  • VDAC1的枯竭改变了瘤的微环境,减少了血管生成.
  • 诱导了表观遗传修饰,包括基因素乙化和甲基化变化,影响了基因表达.

结论:

  • 准VDAC1是一种有前途的抗癌治疗策略,特别是在高能耗癌症中.
  • 通过调节新陈代谢和表观遗传学,VDAC1沉默为抗癌提供了一种新的方法.
  • VDAC1的作用扩展到其他病理,表明更广泛的治疗潜力.