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Updated: Jun 9, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
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同时检测8种乳制品成分使用双管多重复 qPCR 基于 TaqMan 探针

Yingying Su1, Lu Meng1, Jiaqi Wang1

  • 1State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Haidian District, Beijing 100193, China.

Foods (Basel, Switzerland)
|October 26, 2024
PubMed
概括

使用多重实时聚合酶链反应 (PCR) 的新方法检测乳制品中的动物DNA. 这种高通量选在商业样本中发现了33%的错误标签率,有助于检测食品欺诈.

关键词:
在PCR效率方面,PCR效率很高.在 TaqMan 探测器中,伪造 伪造 伪造双管和多重复 qPCRR 的复合.检测的检测极限.牛奶和奶制品的使用

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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