寒冷等离子体对Bacillus cereus细胞造成的潜致伤害:对细胞活力和生物膜形成能力的影响
Laura Eced-Rodríguez1, Michael Beyrer2, Dolores Rodrigo3
1Departmento de Microbiologia y Ecologia, Facultad de Ciencias Biológicas, Universitat de València, 46010 Valencia, Spain.
Foods (Basel, Switzerland)
|October 26, 2024
概括
冷血有效地使食物中的Bacillus cereus群细菌失活. 这种非热技术会破坏细胞并破坏生物膜,提供了一个有前途的食品安全解决方案.
科学领域:
- 食品微生物学和食品安全
- 非热处理技术非热处理技术
- 等离子体科学与工程 等离子体科学与工程
背景情况:
- 在粉末和粉类食品中,Bacillus cereus集团构成显著的风险.
- 冷等离子体是一种新兴的非热技术,用于食品巴氏杀菌.
- 了解冷等离子体的失活机制对于其应用至关重要.
研究的目的:
- 评估冷血对Bacillus cereus无活化的机制.
- 评估冷等离子体在形态和分子层面上损害细菌细胞的能力.
- 研究冷血对缓解Bacillus cereus生物膜的有效性.
主要方法:
- 介电屏障放电冷等离子体 (DBD-CP) 用于无活化.
- B. cereus,B. thuringiensis和B. mycoides在不同的功率 (100-300W) 和时间 (1-10分钟) 中进行了处理.
- 评估了细胞损伤 (形态,分子),DNA/蛋白质泄漏和生物膜形成能力.
主要成果:
- 在DBD-CP中,Bacillus物种实现了2-7 log10的无活化周期.
- 观察到亚致命性损伤 (45-98%),包括细胞膜孔隙和DNA/蛋白质泄漏.
- 冷血治疗逐渐分解了B. cereus生物膜,并降低了受损细胞的生物膜形成能力.
结论:
- 冷血有效地使Bacillus cereus群细菌失活,并破坏它们的生物膜.
- 寒冷等离子体诱导的次致命损伤在减少生物膜形成中起作用.
- 量身定制的DBD-CP条件可以提高食品安全应用的无活化和生物膜减少.
更多相关视频
相关概念视频
Physical Methods for Controlling Microbial Growth: Temperature
1
Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
1
Biological Methods for Microbial Control
2
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
2
Biofilms
1
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
1


