红藻中多功能脂氧酶的复杂演变 红藻中的多功能脂氧酶
Zhujun Zhu1,2, Yanrong Li1, Xinru Wu2
1Marine Drugs and Biological Products Department, Ningbo Institute of Oceanography, Ningbo 315832, China.
International journal of molecular sciences
|October 26, 2024
概括
红藻脂氧酶 (LOXs) 进化了多功能脂肪酸氧化能力. 在PhLOX中的关键氨基酸突变和域收购解释了其组合的氧化酶和氧化物合成酶活动.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 酶学 是一种酶学.
背景情况:
- 下层有机体的脂氧酶 (LOXs) 在脂肪酸氧化中表现出多样化的基质和功能多样性.
- 在LOX中使多功能催化域成为可能的进化机制仍然不清楚.
研究的目的:
- 研究红藻 *Pyropia haitanensis* (PhLOX) 中多功能脂氧酶 (LOX) 活性的结构和进化基础.
- 阐明PhLOX如何将氧化酶 (HPL) 和氧化合成酶 (AOS) 活动集成到单个催化域内.
主要方法:
- 分子对接和位点定向突变发生被用来识别关键的氨基酸残留物.
- 进行了遗传学分析,以追踪进化关系.
- 对来自*Shewanella violacea*和*Chondrus crispus*的同类LOX进行了功能分析.
主要成果:
- 特定的残留物 (Phe642, Phe826) 调节脂肪酸过氧化,而其他 (Gln777, Asn575) 则对AOS功能至关重要.
- 在Asn575,Gln777或Phe826中对白的突变增强了HPL活性.
- 遗传学分析揭示了PhLOX分类中的独特地点 (Asn575,Phe826) 和其他LOX中的保存残留物 (Phe642,Gln777).
- N端的SRPBCC域对于PhLOX的功能多功能性至关重要.
- HPL活动似乎是祖先的,而AOS功能是通过活性口袋突变获得的.
结论:
- 菲洛克斯通过祖先的功能域和独特的氨基酸替代的组合实现了其双重的HPL和AOS活动.
- 红藻LOXs通过整合现有域和在活跃地点内获得特定突变来进化了多功能性.
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