基于时间序列转录组数据的基础上,在土壤盐度压力下的"Jao Khao"大米的双态权重基因共同表达网络中对关键枢纽基因进行概括
Prasit Khunsanit1,2, Kitiporn Plaimas3, Supachitra Chadchawan4,5
1Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
International journal of molecular sciences
|October 26, 2024
概括
这项研究使用RNA测序来识别盐耐受性大米中的关键基因 (
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 土壤盐化威胁全球农业,迫使开发耐盐作物.
- 识别大米盐耐受性的分子机制对于培育性品种至关重要.
- 了解早期的应激反应可以揭示改善作物在盐水条件下生存的关键遗传标.
研究的目的:
- 在盐度压力下研究耐盐泰国大米品种 ("Jao Khao") 的时间过程转录组.
- 通过使用RNA测序和共同表达网络分析,识别参与早期盐应激反应的关键基因和分子通路.
- 提供候选基因,以提高大米育种计划中的盐耐受性.
主要方法:
- 进行了RNA测序 (RNA-Seq) 在正常和盐水 (160毫米NaCl) 条件下,在48小时内抽取样本的"Jao Khao"大米叶上.
- 使用时间序列转录组分析,差异基因表达分析和加权基因共同表达网络分析 (WGCNA).
- 反转录定量聚合酶链反应 (RT-qPCR) 用于验证关键基因的表达.
主要成果:
- 在盐水条件下",Jao Khao"表现出高盐耐受性和稳定的生理参数.
- 从1950个高度可变的基因中确定了111个关键枢纽基因,这些基因与ATP驱动的运输,光反应,ATP合成和碳固定相关的途径丰富.
- 在最初的盐应激反应期间,观察到能量代谢基因的早期上调,包括RuBisCo和ATP合成酶.
- 差异基因表达分析显示,在盐暴露后的前48小时内,基因活性发生了显著变化.
结论:
- 该研究强调了能源代谢管理在植物盐应激反应早期阶段的关键作用.
- 参与能源生产和利用的关键基因对于赋予大米盐耐受性至关重要.
- 已识别的枢纽基因代表了基因工程和育种策略的有希望的目标,以提高米盐耐受性.
相关概念视频
Responses to Salt Stress
13.0K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.0K
Constitutive and Regulated Gene Expression
1
Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
1
Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
Transgenic Plants
7.2K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.2K
Regulation of Expression at Multiple Steps
873
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
873
mRNA Stability and Gene Expression
5.5K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
5.5K


