对天然蛋膜 (NEM) 的生物活性成分进行剖析,以保护软骨及其对人类红细胞氧化应激的保护作用
Jin-Woo Kim1, Dong-Ho Lee1, Kang-Woo Lee1
1Department of Food Science and Biotechnology, Sejong University, Neungdong-ro 209, Gwangjin-gu, Seoul 05006, Republic of Korea.
International journal of molecular sciences
|October 26, 2024
概括
自然的蛋膜 (NEM) 含有软骨保护性化合物和. 尼姆有效地清除自由基,并减少人体软骨细胞中的氧化应激和亡,这表明骨关节炎治疗的潜力.
科学领域:
- 生物化学 生物化学
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
背景情况:
- 骨关节炎 (OA) 涉及氧化应激和软骨退化.
- 自然蛋膜 (NEM) 是生物活性化合物的潜在来源.
研究的目的:
- 研究NEM的物理化学性质和保护作用,以对抗人类红细胞中氧化应激的作用.
- 在NEM中识别生物活性成分和抗氧化.
主要方法:
- 对NEM生物活性成分 (氨酸,氏素硫酸,原蛋白,蛋白质) 的分析.
- 在体外胃肠道消化和纳米LC-ESI-MS分析用于的识别.
- 在H2O2处理的SW-1353细胞中,ABTS检测激素清除活性和氧化应激标记物 (SOD,ROS) 的评估.
- 细胞实时成像以评估细胞亡.
主要成果:
- 尼姆含有大量的氨酸,氏丁硫酸盐,原蛋白和蛋白质.
- 在NEM中确定了特定的抗氧化 (TEW,SWVE,VYL).
- 与阳性对照相比,NEM表现出更高的激素清除活性.
- 根据治疗前的剂量,NEM降低了ROS水平,增强了SOD表达,并降低了细胞的亡.
结论:
- 尼姆含有丰富的软骨保护成分,并具有强大的抗氧化和抗丧性质.
- 通过减轻氧化应激,NEM显示出作为治疗关节炎的治疗剂的潜力.
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